FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310271
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA70022.256736852622385Illumina PCR Primer Index 11 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69852.2512577714320705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42391.3662250097495416No Hit
TTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA34121.0996838247854295RNA PCR Primer, Index 45 (96% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30650.9878461087243088No Hit
ATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA27080.872785403727709Illumina PCR Primer Index 11 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16500.5317931743540325No Hit
TATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA11610.3741890154091101Illumina PCR Primer Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA8720.28104463517376743Illumina PCR Primer Index 11 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.19982531399969702No Hit
CTTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA4570.1472905943513896Illumina PCR Primer Index 11 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.1453568009901022No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.12279587844174931No Hit
CTTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA3550.11441610720950394Illumina PCR Primer Index 11 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3380.10893702601918967No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT3260.1050694392966149No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)