FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002151084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991112
Sequences flagged as poor quality0
Sequence length100
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363933.6719361686671137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338073.411017120164018No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282832.853663359943175No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171791.7333056203537038No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT162371.6382608625463115Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159511.6094043861844072No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146921.482375352129729No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA128961.3011647523186076Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG120821.2190347811347253Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT103841.0477120648322287Clontech SMART CDS Primer II A (100% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCATTAATCATCTCGTATGCCGT90990.9180597147446505No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA86600.8737660324968319No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA85220.8598422781683605Clontech Universal Primer Mix Long (95% over 21bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT78870.7957728289032925Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77460.7815463842633324No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG67700.6830711362590707Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66640.6723760785864766No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT56480.5698649597623678Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55430.5592707988602701Clontech SMART CDS Primer II A (100% over 21bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT51260.5171968455633672Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT51140.5159860843174132Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT48750.49187175616882856Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA46890.4731049568565409Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT46840.47260047300406005Clontech SMART CDS Primer II A (100% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT45820.46230900241345074Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCATTAATCATCTCGTATGCCGTCTT45450.45857582190509244TruSeq Adapter, Index 7 (95% over 21bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTT41570.41942787495257855Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTT38760.3910758824431548No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37640.37977544414758374Clontech SMARTer II A Oligonucleotide (95% over 21bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC36210.3653472059666314Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT35120.35434945798254885Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACAC33640.33941673594911576No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATACAC33450.3374996973096885No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA33390.3368943166867115Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT33230.3352799683587728Clontech SMART CDS Primer II A (100% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACA31790.3207508334073243No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31720.3200445560138511Clontech SMART CDS Primer II A (100% over 23bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA31030.3130826788496154No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA30870.31146833052167666No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATACA30060.30329569211148694No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC29990.3025894147180137Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT28100.28351992509423757Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC27800.2804930219793525Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA27550.27797060271694823Clontech Universal Primer Mix Long (95% over 22bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCT27340.2758517705365287Clontech Universal Primer Mix Long (95% over 23bp)
CATCTCCGAGCCCACGAGACCATTAATCATCTCGTATGCCGTCTTCTGCT27150.27393473189710144TruSeq Adapter, Index 7 (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT26960.27201769325767416Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC26490.2672755450443542Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGCTGTCTCT26410.2664683708803849No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25450.2567822809127525No Hit
ACACATCTCCGAGCCCACGAGACCATTAATCATCTCGTATGCCGTCTTCT25270.2549661390438215RNA PCR Primer, Index 7 (95% over 23bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24180.24396839105973897No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG22930.23135629474771774Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22320.22520159174745136No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACAC21880.22076213384561988No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACAC21560.21753343718974244No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC21310.2150110179273382Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21030.21218590835344545Clontech Universal Primer Mix Long (95% over 21bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC20880.21067245679600288Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20720.20905810846806416Clontech Universal Primer Mix Long (95% over 22bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTG20660.20845272784508714Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTT20390.20572851504169054No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTG20030.20209623130382845Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA19850.20028008943489736Clontech Universal Primer Mix Long (95% over 22bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT19560.19735408309050845Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTATA19190.19362090258215015No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA19000.19170386394272293No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA18860.19029130915577655Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTC18660.18827337374585315Clontech Universal Primer Mix Long (100% over 23bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTC18600.18766799312287613Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA17820.17979804502417487Clontech Universal Primer Mix Long (95% over 22bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA17640.17798190315524381Clontech Universal Primer Mix Long (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGT17560.17717472899127443Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGT17520.17677114190928978Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAA17320.17475320649936638Clontech Universal Primer Mix Long (95% over 22bp)
TCTCCGAGCCCACGAGACCATTAATCATCTCGTATGCCGTCTTCTGCTTG16870.1702128518270387TruSeq Adapter, Index 7 (96% over 28bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACA16850.17001105828604637No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT16770.16920388412207701No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATACACA16720.16869940026959618No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTT16620.16769043256463445Clontech Universal Primer Mix Long (95% over 23bp)
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16430.16577339392520724No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACAT16320.16466352944974938No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATAC16240.16385635528578002No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACAT16130.16274649081032214No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA16120.16264559403982595Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATCT15850.15992138123642938No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTT15690.15830703290849066No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGCTGTCTCTT15530.15669268458055197Clontech Universal Primer Mix Long (95% over 23bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA15470.15608730395757492Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT14990.15124425897375876Clontech Universal Primer Mix Long (100% over 23bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATAC14900.15033618803929324No Hit
TTATACACATCTCCGAGCCCACGAGACCATTAATCATCTCGTATGCCGTC14340.1446859688915077No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC14010.1413563754651341Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTATA13530.13651333048131797Clontech Universal Primer Mix Long (95% over 21bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATCTGTCTCTTATA13440.13560525954685243Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC13210.1332846338254405Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTT13130.13247745966147115No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13120.13237656289097496Clontech SMART CDS Primer II A (100% over 22bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGT13120.13237656289097496Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACA13090.13207387257948647No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGT12880.1299550403990669Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC12730.12844158884162435Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12500.12612096312021245No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATACA12310.12420392448078522No Hit
GCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA12290.12400213093979287No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA12140.12248867938235034Clontech Universal Primer Mix Long (95% over 22bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTAT12130.12238778261185415No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTGTCTCTTAT12020.1212779181363963No Hit
GTATCAACGCAGAGTACATGGGAAGCAGCTGTCTCTTATACACATCTCCG11980.1208743310544116No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11900.12006715689044224Clontech SMART CDS Primer II A (100% over 24bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC11810.11915908595597673Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG11720.1182510150215112No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGCTGTCT11680.1178474279395265Clontech Universal Primer Mix Long (95% over 22bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATACACATCTCC11650.11754473762803799No Hit
GGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACACATCTCCGA11650.11754473762803799No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11630.11734294408704565No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCT11590.11693935700506099Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTCT11580.11683846023456482Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTAT11530.11633397638208397No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCT11520.1162330796115878Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC11490.11593038930009927No Hit
GATACCACTGCTTCCCATGTACTCTGCGTCTGTCTCTTATACACATCTCC11340.11441693774265674No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT11340.11441693774265674No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTC11260.11360976357868736Clontech Universal Primer Mix Long (95% over 23bp)
GCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG11210.11310527972620651No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTATAC11140.11239900233273334No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11120.11219720879174098Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATC11080.11179362170975632No Hit
GTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACACATCTCCGAG11030.11128913785727547No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTT10950.11048196369330611No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATC10930.11028017015231376No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10830.10927120244735207Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC10830.10927120244735207No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGCTGTCTCTTATACACATCTCC10820.10917030567685589No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACACA10650.107455060578421Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGCTGTCTC10400.10493264131601675No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTT10330.10422636392254357No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATACACA10220.1031164994470857Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCTCTTATACACA10090.10180484143063549Clontech Universal Primer Mix Long (95% over 21bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGT10070.10160304788964314Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTCTTATACACA9920.1000895963322006Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)