FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002151085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991112
Sequences flagged as poor quality0
Sequence length100
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186961.8863660211963937No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176361.7794154444704533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153941.5532048850180402No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT141421.4268821283568356Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG106771.0772748185876067Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100141.010380259748646No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT89820.9062547925965985Clontech SMART CDS Primer II A (100% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA80570.8129252798876413Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80450.8117145186416872No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79100.7980934546247045No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA77940.7863894292471486No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT69740.7036540774402893Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA60680.6122416033707594Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG59540.6007393715341959Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT48970.49409148511974427Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT47520.47946145339779966Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT46520.4693717763481826Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT44660.450604977035895Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT41320.4169054556901743Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA39860.4021745271977335Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT39070.39420368232853603Clontech SMART CDS Primer II A (100% over 26bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38620.38966332765620837No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTT38040.38381131496743054No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTT35630.35949519327785356Clontech Universal Primer Mix Long (95% over 21bp)
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35040.35354228381857955No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32540.32831809119453703No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC32180.3246858074566749Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT31980.3226678720467515Clontech SMART CDS Primer II A (100% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACAC31550.3183293109154162No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATACAC31330.31610958196450045No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT30810.31086294989869967Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATACA29470.29734278265221287No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA28940.29199525381591585No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA28830.290885389340458No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACA28240.2849324798811839No Hit
ATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28090.28341902832374144No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26910.27151320940519336Clontech SMART CDS Primer II A (100% over 21bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC26690.26929348045427765Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT25980.26212980974904954Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGCTGTCTCT25000.2522419262404249No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT24930.25153564884695173Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC24810.2503248876009977Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCT24780.2500221972895092Clontech Universal Primer Mix Long (95% over 23bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC24590.24810515865008195Clontech Universal Primer Mix Long (96% over 26bp)
GTAAATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA24430.24649081032214323Clontech Universal Primer Mix Long (95% over 21bp)
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22570.2277240110098556Clontech SMARTer II A Oligonucleotide (95% over 21bp)
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20980.21168142450096455No Hit
ATACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20310.2049213408777212Clontech Universal Primer Mix Long (95% over 21bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACAC20300.204820444107225No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACAC20140.20320609577928628No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG19670.1984639475659663Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTG18880.1904931026967689Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTG18770.189383238221311Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC18330.18494378031947953Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC17930.18090790949963273Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA17810.1796971482536787No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17690.17848638700772465Clontech SMART CDS Primer II A (100% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTATA17620.17778010961425145No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTC17500.17656934836829744Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT16960.17112092276150426Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTC16880.1703137485975349Clontech Universal Primer Mix Long (100% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA16780.1693047808925732Clontech Universal Primer Mix Long (95% over 22bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGT16260.16405814882677233Clontech Universal Primer Mix Long (96% over 26bp)
CAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15980.16123303925287957No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACA15640.15780254905600982No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGT15630.15770165228551364Clontech Universal Primer Mix Long (96% over 26bp)
ATAAATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA15600.15739896197402514Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATCT15520.15659178781005578No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATACACA15470.15608730395757492No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATAC15350.15487654271162088No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACAT15330.15467474917062854No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACAT15150.15285860730169748No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGCTGTCTCTT15010.1514460525147511Clontech Universal Primer Mix Long (95% over 23bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT14750.14882273648185068No Hit
AAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14730.14862094294085834No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA14520.1465021107604388Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTT14290.14418148503902686Clontech Universal Primer Mix Long (95% over 23bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATAC14120.14246623994059196No Hit
AAAGCAGTAGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14000.14125547869463795No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA13870.13994382067818772Clontech Universal Primer Mix Long (95% over 22bp)
AAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA13700.13822857557975285Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13700.13822857557975285Clontech Universal Primer Mix Long (95% over 21bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13310.13429360153040223Clontech Universal Primer Mix Long (95% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT13310.13429360153040223Clontech Universal Primer Mix Long (100% over 23bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13310.13429360153040223No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA13300.13419270475990605Clontech Universal Primer Mix Long (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC13080.1319729758089903Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13020.1313675951860133No Hit
AAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12920.1303586274810516Clontech SMARTer II A Oligonucleotide (95% over 21bp)
AGTATCAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12840.12955145331708223No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATCTGTCTCTTATA12620.12733172436616647Clontech Universal Primer Mix Long (95% over 21bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTATA12590.127029034054678Clontech Universal Primer Mix Long (95% over 21bp)
AGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12370.12480930510376223Clontech Universal Primer Mix Long (95% over 21bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC12350.12460751156276989Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAA12200.12309406000532735Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATACA12140.12248867938235034No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGT11810.11915908595597673Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGT11710.11815011825101501Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC11560.11663666669357248Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACA11390.11492142159513759No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGAC11350.1145178345131529No Hit
GGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACACATCTGACG11320.11421514420166438No Hit
GTATCAACGCAGAGTACATGGGAAGCAGCTGTCTCTTATACACATCTGAC11190.11290348618521419No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTAT11120.11219720879174098No Hit
GATACCACTGCTTCCCATGTACTCTGCGTCTGTCTCTTATACACATCTGA11110.11209631202124483No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11100.11199541525074865No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTGTCTCTTAT11100.11199541525074865No Hit
GCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACG11000.11098644754578696No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCT10970.11068375723429845Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCT10890.10987658307032909Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCCTGTCTCTTATACACATCTGA10710.10806044120139802No Hit
GTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACACATCTGACGC10700.10795954443090186No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTATAC10590.10684967995544399No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTCT10530.10624429933246697Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT10530.10624429933246697No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10510.10604250579147462Clontech Universal Primer Mix Long (95% over 22bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATC10500.10594160902097846No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGCTGTCT10370.10462995100452824Clontech Universal Primer Mix Long (95% over 22bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGCTGTCTC10340.10432726069303974No Hit
GCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGC10330.10422636392254357No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10190.10281380913559719Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTAT10150.1024102220536125No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGA10140.10230932528311634No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATC10120.10210753174212399No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC10120.10210753174212399No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGCTGTCTCTTATACACATCTGA10090.10180484143063549No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCTCTTATACACA10040.10130035757815463Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGAATCAACGCAAAAAAAAAAAAAAAAAAAAAAA9940.10029138987319294No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)