FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369993
Sequences flagged as poor quality0
Sequence length20-100
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250676.774993040408872No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46931.268402375180071No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24720.6681207482303719No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23470.6343363252818297No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15000.4054130753825072No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13480.3643312170770798No Hit
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11010.2975731973307603No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10870.2937893419605236No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA9150.24730197598332943No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC8410.22730159759779242No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG8280.223788017611144No Hit
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7920.2140581038019638No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7700.2081120453630204Clontech Universal Primer Mix Long (96% over 26bp)
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA7070.1910846961969551No Hit
ATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6790.1835169854564816No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT6180.16703018705759298No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG6120.16540853475606296No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT6050.1635166070709446No Hit
ATGGGAAGCAGTGGTATCAACGC5210.1408134748495242No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC5120.13838099639722912No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT4930.1332457641090507No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4580.12378612568345888No Hit
ATGGGGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4520.12216447338192886No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG4430.1197319949296338Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG4210.11378593649069037No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG4180.11297511033992536No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC4050.10946153035327696No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT4030.10892097958610028No Hit
ATGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3960.1070290519009819No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA3870.10459657344868688No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA3820.10324519653074518No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3790.10243437037998017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)