FastQCFastQC Report
Wed 8 May 2024
EGAF00002151152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580066
Total Bases50 Mbp
Sequences flagged as poor quality0
Sequence length20-100
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65611.1310781876545082No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG12520.2158375081456248No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10280.17722121275854816No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT8480.14619026110821873No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA7110.12257225901880131No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC7000.12067592308461451No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG6670.11498691528205411No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6660.11481452110621895No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT6640.11446973275454862No Hit
GTCTTAGACAGAGTGAGCTCATCCAGCACCCGGCGCAGGCCGTTGATGTC6540.11274579099619698No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA6420.11067706088617503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)