FastQCFastQC Report
Wed 8 May 2024
EGAF00002151286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381319
Total Bases33.2 Mbp
Sequences flagged as poor quality0
Sequence length20-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198125.1956498364886095No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC58971.5464742118803416No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT29690.7786131821388391No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT25790.6763366105544176No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA25200.6608640010070309No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC19260.5050889150553736No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA18500.4851580959773838No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC18080.47414369596059985No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT17110.44870567687421814No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG16230.42562788636286153No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG15660.41067977205436923No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC15320.4017633529931632No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG14960.39232243869306277No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT14370.37684982914567594No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT13770.36111497197884185No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC12550.3291207624062793No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA12530.32859626716738477No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG12090.31705737191170646No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC12020.31522163857557584No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA11540.3026337528421086Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC11480.30106026712542516No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG11360.2979132956920583No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA11330.29712655283371664No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA10870.28506316233914386No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT10640.2790314670918575No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT10410.2729997718445711No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA9930.26041188611110383No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT9370.24572601942205868No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC8610.22579520034406889No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG8510.22317272414959655No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGAT8510.22317272414959655No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA8450.22159923843291315No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG8230.21582979080507397No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG8170.21425630508839058No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7980.20927360031889308Clontech Universal Primer Mix Long (96% over 26bp)
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT7500.19668571458542586No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCC7460.1956367241076369No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7390.19380099077150628No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT7300.19144076219648115No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG7260.19039177171869223No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA7140.18724480028532542No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC7120.18672030504643095No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGG7120.18672030504643095No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC7080.185671314568642No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC7010.18383558123251137No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC7010.18383558123251137No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATA7000.18357333361306413No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGTGCATTTT6940.1819998478963807No Hit
GAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT6920.18147535265748627No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC6900.1809508574185918No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGA6700.1757059050296471No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT6670.17491916217130538No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT6530.1712476954990441No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG6390.16757622882678283No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA6210.16285577167673262No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTC6170.16180678119894365No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGG6160.16154453357949644No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG6110.16023329548226026No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG6000.15734857166834068No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTA5930.15551283833221005No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTTG5780.15157912404050153No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACATACTGAT5700.14948114308492363No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5590.14659641927100406No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTG5570.1460719240321096No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA5360.14056472402371767No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTT5340.1400402287848232No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCG5240.13741775259035086No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTT5160.135319771634773No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCG5150.13505752401532575No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCG5130.13453302877643128No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTT5040.13217280020140618No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTT5020.1316483049625117No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTT5010.13138605734306447No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGT4960.1300748192458283No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGC4940.12955032400693384No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGT4920.12902582876803936No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC4900.1285013335291449No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCC4890.12823908590969765No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG4860.12745234305135594No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4820.12640335257356702No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC4740.12430537161798914No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTT4740.12430537161798914No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACC4680.12273188590130574No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4530.11879817160959721No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA4500.11801142875125552No Hit
AGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCA4490.11774918113180827No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTG4460.11696243827346657No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAAC4370.11460220969844147No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT4340.11381546684009977No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTT4320.11329097160120528No Hit
GGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACAC4270.11197973350396911No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC4240.11119299064562742No Hit
ACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCG4220.11066849540673294No Hit
GCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4220.11066849540673294No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATG4210.1104062477872857No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCT4210.1104062477872857No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCC4180.10961950492894401No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4180.10961950492894401No Hit
CGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGC4090.1072592763539189No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4070.10673478111502444No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGA4040.10594803825668273No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACC4040.10594803825668273No Hit
GTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGCA3970.10411230492055208No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA3900.10227657158442144No Hit
CCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTG3900.10227657158442144No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT3890.10201432396497422No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)