FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002151378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188532
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG46092.4446778265758597Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG30971.642691956803089Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT19251.021046824942185Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC15570.8258544968493412Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC14650.7770564148261303Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11610.6158105785755204Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10940.5802728449281819Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10800.5728470498376934Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG8750.46411219315553853Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG8300.44024356607896803Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT7300.3872021725754779Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6900.36598561517408185Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC6900.36598561517408185Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC6580.349012369252965Clontech Universal Primer Mix Long (96% over 26bp)
ACCTACACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTTGG6530.3463602995777905No Hit
GGTGTAGGTCACGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT5530.2933189060743004No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT5490.2911972503341608Clontech SMART CDS Primer II A (96% over 26bp)
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGG5290.28058897163346275No Hit
CCCTAACGTTGCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCGAAGCTCGCGCGAAA5120.27157193473786945No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4900.25990282816710164Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.2583115863619969No Hit
AGGTAGGACGGCGCGGCTGCTTTGTTGAGCCGTGCCACGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGC4520.23974709863577537No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC4510.23921668470074045Clontech Universal Primer Mix Long (96% over 26bp)
GTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCTCCCACGCCCGGTCGTACTCATAAC4240.22489550845479814No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG4160.2206521969745189Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTGAGTTGGCTGTTCGACGCCCGGGGAAGGCCCCCGAAAGAGCCGTTCCCAGTCCGTCCCCCGGCCGGCACG4000.2121655740139605No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT3940.2089830904037511Clontech Universal Primer Mix Long (96% over 26bp)
GTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCCCGGGTGCGAGTTCTATCGGGTAAAGCG3870.20527019285850678No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTTGGGCGTGAGCCTGGGCGGAGCGGCCGTCGGTGCGGATCTTG3790.20102688137822758No Hit
GAGTACGACCGGGCGTGGGAGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCGCGC3780.20049646744319266No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG3770.19996605350815774Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCT3720.19731398383298326No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGG3720.19731398383298326No Hit
CTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCG3670.19466191415780876No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGAAAGCAGCTCGAGCAGTCCGCCGACAG3590.19041860267752955No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3540.18776653300235502Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT3490.18511446332718054No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC3470.18405363545711073Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC3460.18352322152207584Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTACTCATCGGGGCCTGGCACTTGCCCCGACGGCCGGGTGTAGGTCACGCGCTTAAGCGCCATCCATTTTCG3420.18140156578193622No Hit
GTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGAC3410.1808711518469013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.17874949610676172No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGAGGCACTCGGTCCTCCGG3290.1745061846264825No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT3210.1702628731462033Clontech Universal Primer Mix Long (96% over 25bp)
CCCTGGAAACGACTCAGTCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCGCACGTCGCGCGGTGTCCGGTGCGC3170.1681412174060637No Hit
CCCCTTGTCCGTACCAGTTCTGAGTTGGCTGTTCGACGCCCGGGGAAGGCCCCCGAAAGAGCCGTTCCCAGTCCG3160.1676108034710288No Hit
GTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGACATTGCGTCCCCGATGCCTCTAATCATTCGCTTTACCCG3160.1676108034710288No Hit
GTGCCAAACTACGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAA3150.16708038953599388No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.166549975600959No Hit
GTCCTCGACCTATTCTCAAACTTTAAATAGGTAGGACGGCGCGGCTGCTTTGTTGAGCCGTGCCACGGAATCGAG3120.16548914773088919No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCGGACTCCCT3100.16442831986081938No Hit
ATCCTAAGAGACGGGGGAAGCCCGTCAGATAGCGCGTTTCGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTCC3070.16283707805571468No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT3070.16283707805571468Clontech SMART CDS Primer II A (100% over 26bp)
TCTCCGGACTCCCTAACGTTGCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCGAAGC2910.15435045509515627No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2890.15328962722508646Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC2890.15328962722508646Clontech SMART CDS Primer II A (96% over 26bp)
TTGTTGAGCCGTGCCACGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGA2840.15063755754991195No Hit
GCGCTTAAGCGCGTGACCTACACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGGCGCGGCGGTC2820.14957672967984215No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTTAAGCGCGTGACCTACACCCGGC2800.14851590180977234No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACT2790.14798548787473745No Hit
GTTTGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGC2760.14639424606963275No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTC2760.14639424606963275No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTTCTAGG2740.14533341819956294No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2720.14427259032949313Clontech Universal Primer Mix Long (96% over 26bp)
GTCCGTACCAGTTCTGAGTTGGCTGTTCGACGCCCGGGGAAGGCCCCCGAAAGAGCCGTTCCCAGTCCGTCCCCC2710.14374217639445824No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTA2680.14215093458935352No Hit
GTGCTCTTCCAGCCGCTGGACCCTACCTCCGACTGAGTCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGAT2660.14109010671928374No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTTAAGCGCGTGACCTACACCCGGCCG2660.14109010671928374No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA2610.13843803704410923Clontech Universal Primer Mix Long (96% over 26bp)
ACCTCCGACTGAGTCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGA2600.1379076231090743No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTTAAGCGCGTGACCTA2590.13737720917403942No Hit
AGCCTGGGCGGAGCGGCCGTCGGTGCGGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGA2580.13684679523900453No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2540.13472513949886492Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGG2530.13419472556383No Hit
GCCCAGGCTCACGCCCAAGGTTTTGCAGCGACCGCCGCGCCCTCCTACTCATCGGGGCCTGGCACTTGCCCCGAC2520.1336643116287951No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA2510.13313389769376022Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG2460.1304818280185857Clontech Universal Primer Mix Long (96% over 26bp)
GTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCTGGAAACGACT2440.1294210001485159No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGGCTGTTCGACGCCC2360.1251776886682367No Hit
CCGGACACCGCGCGACGTGCGGTGCTCTTCCAGCCGCTGGACCCTACCTCCGACTGAGTCGTTTCCAGGGTGGGC2350.1246472747332018No Hit
CCCTCCTACTCATCGGGGCCTGGCACTTGCCCCGACGGCCGGGTGTAGGTCACGCGCTTAAGCGCCATCCATTTT2350.1246472747332018No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT2210.11722147964271316Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.11616065177264337No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTTGGGCGTGAGCCTGGGCGGAGCGGCCGTCGGTGCGGATCTTGGT2180.11563023783760847No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2160.11456940996753867Clontech Universal Primer Mix Long (96% over 25bp)
GTTATGAGTACGACCGGGCGTGGGAGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACAC2140.11350858209746888No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC2110.11191734029236416Clontech Universal Primer Mix Long (96% over 25bp)
ACCGGACACCGCGCGACGTGCGGTGCTCTTCCAGCCGCTGGACCCTACCTCCGACTGAGTCGTTTCCAGGGTGGG2100.11138692635732925No Hit
GTACATGGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAAC2070.10979568455222455No Hit
CCTTTGAGGGGTGCACCGCCGACCGACCTTGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCC2060.10926527061718966No Hit
GTCGAGGACATTGCGTCCCCGATGCCTCTAATCATTCGCTTTACCCGATAGAACTCGCACCCGGGCTCCAGCTAT2040.10820444274711985No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT2030.10767402881208495Clontech Universal Primer Mix Long (96% over 25bp)
GTCCCGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTTTCGCGGCGAGAGCGGGTCGCCGCGTGCCGGCCG2020.10714361487705006No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGG2010.10661320094201515No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGTAGTTTGGCACCGTAACCCGGCTTCCGGTTCA2000.10608278700698025No Hit
GTCCGCCGACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAGGTT1990.10555237307194536No Hit
CACCGGACACCGCGCGACGTGCGGTGCTCTTCCAGCCGCTGGACCCTACCTCCGACTGAGTCGTTTCCAGGGTGG1980.10502195913691045No Hit
CCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCC1980.10502195913691045No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1980.10502195913691045Clontech Universal Primer Mix Long (96% over 26bp)
GCTCAACAAAGCAGCCGCGCCGTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGACATTGCGTCCCCGATGCC1970.10449154520187555No Hit
GTCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCGCACGTCGCGCGGTGTCCGGTGCGCCCCCGGCGGCCCTTGA1970.10449154520187555No Hit
GTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCTCCCACGCCCGGTCGTACTCATAACCG1950.10343071733180573No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT1940.10290030339677084Clontech Universal Primer Mix Long (96% over 26bp)
GTGCGGATTGGTGCGGCGGCCAAAGCCCGGGCTGTTGATATGCCCGTGGAGATGCCGTCGCGTCGATCGTGGTTG1940.10290030339677084No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGGCTGTTCGA1940.10290030339677084No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGT1930.10236988946173593No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA1920.10183947552670103Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)