FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002151382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205898
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13510.6561501325899232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.4390523463073949No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6840.33220332397594926No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGG4900.23798191337458352No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGAACGGGCGCAA4860.23603920387764815No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.21126965779172213No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3980.1932995949450699Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG3790.18407172483462686Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT3370.1636732751168054No Hit
GTTAAGGACGCACCGGATGCAACAGAAGTGCTGCACTTTGCCCGTCATTGAACCTTATCTCCGGATAATCCGATT3320.16124488824563618No Hit
GTCCTTAACAGTCCTTGAAAAACCGATGGAACGTATTACTCTCATGCCTGGTCGTACTCATAACCGCAGCAGGTC3110.15104566338672545No Hit
GTCCCAGCCAGACCCACTCCAGTTGCCCAGAGCAGTTCCAGCCGGGTCCAAACCCTTCCAGCCAAGGCCCGATGC2960.1437605027732178No Hit
GTTCCAGCCGGGTCCAAACCCTTCCAGCCAAGGCCCGATGCACCCAACCCTTAGAGCCAATCCTTATCCCGAAGT2770.13453263266277476No Hit
GTTCTGAGCTGGCTGTTCGACGCCCGGAGAAGGCTCCCGAAGAAGCCATTCCCAGTCCGTCCCTCGGCCAGCACG2750.13356127791430708No Hit
CCCCTTGTCCGTACCAGTTCTGAGCTGGCTGTTCGACGCCCGGAGAAGGCTCCCGAAGAAGCCATTCCCAGTCCG2700.13113289104313786No Hit
GTCTATAACCTTGAAATCGGATTATCCGGAGATAAGGTTCAATGACGGGCAAAGTGCAGCACTTCTGTTGCATCC2640.1282188267977348No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT2570.12481908517809791No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGAGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCT2530.12287637568116253No Hit
GTTCTAAGTTGGTTGTTAATTGTACACCAGACGCCCAAAGGCAGCCTAAGCCTTTCAAGCTCAGTCCCAGCCAGA2480.1204479888099933No Hit
TTCCCAGTCCGTCCCTCGGCCAGCACGTGGCAACCCGCTCTCGCCGCGGAAGCAGCTCGAGCAGTCCGCCGACAG2400.11656256981612255No Hit
GTTATAGACGGTCAAGGAGAAAAGATAACTCTTCCCAGGGCCCTTACCAGCGTCACCAACTTCATTTACGTTACC2300.11170579607378411No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCCCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTTAG2220.10782037707991335No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGTTGGGTGCATCG2180.10587766758297797No Hit
GCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCT2160.10490631283451028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)