FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205898
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGG4290.20835559354631905No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGAACGGGCGCAA3560.17290114522724845No Hit
GTTAAGGACGCACCGGATGCAACAGAAGTGCTGCACTTTGCCCGTCATTGAACCTTATCTCCGGATAATCCGATT3100.15055998601249163No Hit
GTCCCAGCCAGACCCACTCCAGTTGCCCAGAGCAGTTCCAGCCGGGTCCAAACCCTTCCAGCCAAGGCCCGATGC2890.1403607611535809No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2760.13404695528854094Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.13064721366890403No Hit
GCGTTAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT2570.12481908517809791No Hit
GTCCTTAACAGTCCTTGAAAAACCGATGGAACGTATTACTCTCATGCCTGGTCGTACTCATAACCGCAGCAGGTC2460.11947663406152562No Hit
GTTCTAAGTTGGTTGTTAATTGTACACCAGACGCCCAAAGGCAGCCTAAGCCTTTCAAGCTCAGTCCCAGCCAGA2410.1170482471903564No Hit
GTCTATAACCTTGAAATCGGATTATCCGGAGATAAGGTTCAATGACGGGCAAAGTGCAGCACTTCTGTTGCATCC2370.11510553769342102No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGAGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCT2350.11413418294495332No Hit
TTCCCAGTCCGTCCCTCGGCCAGCACGTGGCAACCCGCTCTCGCCGCGGAAGCAGCTCGAGCAGTCCGCCGACAG2300.11170579607378411No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT2260.10976308657684873No Hit
GCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCT2260.10976308657684873No Hit
GTTCCAGCCGGGTCCAAACCCTTCCAGCCAAGGCCCGATGCACCCAACCCTTAGAGCCAATCCTTATCCCGAAGT2210.10733469970567952No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCCCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTTAG2160.10490631283451028No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG2150.10442063546027644Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)