FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319414
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTA12260.38382788481406577No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAAGCACGTGAAATTGTTAAAAGGGAAG12100.3788187117659213No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTA11020.34500679369094656No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT8490.265799244867163No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA7960.24920635914518463No Hit
CATCAGGATCGGTCGATGATGCACCTTGCGGTTCTCACCTCCGTTCACTTTCATTACGCGTAGGGGTTTGACACC7680.24044030631093188No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATA7520.2354311332627875No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGG7510.23511805994727844No Hit
GCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCA7040.2204036141183542No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC6970.21821210090979104No Hit
GTTATAGCCTCTTGTGATGCAGCGAGCGCCGGGCGAGGTCCGCGCTTCGGCTAGGATGCTGGCGTAATGGTCGTA6890.21570751438571883No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT6820.21351600117715563No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACCAA6530.20443687502739394No Hit
GGCTATAACACTCCCGAGGGAGCTACATTCCTCAAGTCTTATCCAGCGGCACCAGACGATGCTGGCCTGCAACGC6270.19629696882415923No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAAT6240.19535774887763216No Hit
GTGATAGACAGTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGCCGACGTCTCCACCTTCAGTTGCGTTGCC6140.19222701572254192No Hit
CTTGTGCGCCGTCCCTCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATTTGGATTCTCCACGGCAACGTAA5800.18158252299523503No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGG5780.180956376364217No Hit
GTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT5770.18064330304870796No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGGTGTCTGAT5730.17939100978667183No Hit
CTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAA5640.17657334994709062No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGTCC5610.17563413000056355No Hit
GCCTTGGGGGTGACCCCGGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAAC5570.17438183673852745No Hit
GGTCTACTCGCCGCGTTGCAGGCCAGCATCGTCTGGTGCCGCTGGATAAGACTTGAGGAATGTAGCTCCCTCGGG5540.17344261679200035No Hit
GCACCGGACAGCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGA5430.16999881032140107No Hit
CCCCCAAGGCTTCGTCACGGGCCTCCACGCCTGCCTACTCGCTAGGGCATCGTTTCTACCCTAGCGGCGAGGTAT5420.16968573700589204No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGA5350.16749422379732887No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA5320.16655500385080177No Hit
GGTTAAGGTTGTTTCAACCCCAAGGCCTCTAATCATTCGCTTTACCTCATAAAACTGAAAACGTTACTGCTATCC5200.1627981240646935No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC5190.16248505074918446No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGAT5190.16248505074918446No Hit
GGCCAGCATCGTCTGGTGCCGCTGGATAAGACTTGAGGAATGTAGCTCCCTCGGGAGTGTTATAGCCTCTTGTGA5130.1606066108561303No Hit
ACCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTACTTAGTTGAACGTGGACATTTGAATGTAT5020.157162804385531No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG4880.15277977796840464Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGG4850.15184055802187757No Hit
GTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTACTCATAACCG4700.14714445828924216No Hit
GTGCATCATCGACCGATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGT4700.14714445828924216No Hit
CGTCAGAACCGCTGCGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCTTTCG4690.14683138497373316No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACCAATACCTCGCCG4680.14651831165822413No Hit
GAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGTCGAGAGCGCACCGGACAGCACAA4680.14651831165822413No Hit
GTATTGGTAGTACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACACTCCTT4510.14119606529457068No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCC4480.1402568453480436No Hit
GGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAATGATTAGAGGCCTTGGGGTTGAAACAACCTTAACC4470.13994377203253458No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4460.13963069871702558Clontech Universal Primer Mix Long (96% over 26bp)
CTACCAATACCTCGCCGCTAGGGTAGAAACGATGCCCTAGCGAGTAGGCAGGCGTGGAGGCCCGTGACGAAGCCT4460.13963069871702558No Hit
CCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACC4440.1390045520860075No Hit
GCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTAAGAAGAAAAGAGAAC4410.1380653321394804No Hit
ATGATGCACCTTGCGGTTCTCACCTCCGTTCACTTTCATTACGCGTAGGGGTTTGACACCCGAACACTCGCATAG4300.13462152566888114No Hit
GTTCTGACGTGCAAATCGATCGTCAAATTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTC4300.13462152566888114No Hit
GTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACCAATACCTCGC4230.13243001246031796No Hit
GTCTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCTACGCGTAATGAAAGTGAACGGAGGTGAGAACCGCAAG4190.13117771919828186No Hit
ATGCTGGCCTGCAACGCGGCGAGTAGACCGGTCAGAAGACCGGCGGAACACCGCGAGCAAGTCTGGTTGCAATCC4140.12961235262073673No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAACG4080.12773391272768259No Hit
ACCCGGGGTCACCCCCAAGGCTTCGTCACGGGCCTCCACGCCTGCCTACTCGCTAGGGCATCGTTTCTACCCTAG4070.12742083941217355No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAAT4060.12710776609666452No Hit
GTTCTAAGTTGGTTGTTAATTGTACGCCGGACGCCCGAGGGCTGCCAAGACCGTCAGAGACCCGATGCTGGAAGG4050.1267946927811555No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG4030.12616854615013745No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCG4020.12585547283462842No Hit
GAGTAGACCGGTCAGAAGACCGGCGGAACACCGCGAGCAAGTCTGGTTGCAATCCCTTCCCTTTTAACAATTTCA3960.12397703294157426No Hit
GCTCTAGTAACGGCGAGTGAAGCAGCAATAGCTCAAATTTGAAATCTGGCGTCTTCGACGTCCGAGTTGTAATTT3880.12147244641750206No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGG3870.12115937310199303No Hit
ATACGTAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCTTCTAAAGCTA3860.12084629978648401No Hit
ATGCTGGAAGGCTCCGGCCGTGAGGCTAGTGCCGACCTGCTCCAGGTTCGTCCAATCAAGGCCCAACGTACCCAA3860.12084629978648401No Hit
ACCAATACCTCGCCGCTAGGGTAGAAACGATGCCCTAGCGAGTAGGCAGGCGTGGAGGCCCGTGACGAAGCCTTG3850.12053322647097497No Hit
GCGCACAAGTGCGCACTTTAAAACGGAGTTTCCCTATCTCTTAGGATCGACTAACCCATGTCCAACTGCTGTTCA3790.1186547865779208No Hit
GCTCCAGGTTCGTCCAATCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTA3780.11834171326241179No Hit
GCACTAGAGGCCGTTCTACCCGGGGTCACCCCCAAGGCTTCGTCACGGGCCTCCACGCCTGCCTACTCGCTAGGG3770.11802863994690277No Hit
GTCTAGATGAACTAACACCTTTTGTGGTGTCTGATAAGCGTACATTCCGGCACCTTAACCTCGCGTTCGGTTCAT3760.11771556663139375No Hit
CCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGGTGTCTGATAA3730.11677634668486665No Hit
GGTTAGTCGATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAAC3700.11583712673833958No Hit
AGGTTAAGGTGCCGGAATGTACGCTTATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCAGGACGGTGGC3640.11395868684528543No Hit
CCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGC3630.1136456135297764No Hit
GTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTG3630.1136456135297764No Hit
GTCATAGAGGGTGAGAATCCCGTATGCGGTCGGAAAGGCGCTCTATACGTAGCTCCTTCGACGAGTCGAGTTGTT3610.11301946689875836No Hit
CCCTTAAGGGTTCTCACCTCCGTTCACTTTCATTACGCGTATGGGTTTGACACCCAAACACTCGCATAGATGTTA3600.11270639358324933No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAG3560.11145410032121324No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT3540.11082795369019517No Hit
GTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGG3520.1102018070591771No Hit
AGGTAGAGCAGCACACCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTT3520.1102018070591771No Hit
CCACAAAAGGTGTTAGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAAC3510.1098887337436681No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGT3460.10832336716612295No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCTTGAAAGCTATTCCTTCCCGCGG3450.10801029385061395No Hit
GATTACGACCATTACGCCAGCATCCTAGCCGAAGCGCGGACCTCGCCCGGCGCTCGCTGCATCACAAGAGGCTAT3440.10769722053510491No Hit
ACCCCGGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGA3430.10738414721959588No Hit
CCCTTAAGGGTGCATCATCGACCGATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCG3430.10738414721959588No Hit
CTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGGGGAAAGGTTCCG3380.10581878064205076No Hit
AGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAAC3370.10550570732654173No Hit
GGTATTGGTAGTACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACACTCCT3350.10487956069552369No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG3340.10456648738001466No Hit
GTTCTACCCGGGGTCACCCCCAAGGCTTCGTCACGGGCCTCCACGCCTGCCTACTCGCTAGGGCATCGTTTCTAC3330.10425341406450563No Hit
CGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCATTTGAGTAT3270.10237497417145147No Hit
GCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATATTCCGGT3270.10237497417145147No Hit
GGATTATCCGCGGCAACGCAACTGAAGGTGGAGACGTCGGCGGGGGCCCCGGGAAGAGTTCTCTTTTCTTCTTAA3250.1017488275404334No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCC3210.1004965342783973No Hit
CAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCC3200.10018346096288828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)