FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002151414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161378
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16971.0515683674354621No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9450.585581677799948No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7330.4542130897644041No Hit
GTGTACACGTGTTCGTTGAGCAAAGATGCAAATTTAAGCTTTCTCCTCAGCTGAGACAGCAGTGGCGACTGATTG6080.3767551958755221No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.27017313388442044No Hit
GTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTT3870.2398096394799787No Hit
CCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCAC3750.23237368166664601No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG3130.19395456629776053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCAATGATGGGTATTTCTTTTGAAGAGCAGCACGATATTTAGGATGGCTGATACCGTAAACGATTGGATTGTA3120.19333490314664947No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACTGTGGCAGCTC3000.1858989453333168No Hit
GTCCAGAGAGCAGCCCAAACCCAGATGGCAGCAATTTTTCCCATGGCACCTCCGCTTGTCAATGGCTTGCCTGCG2830.17536467176442885No Hit
TCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCA2750.17040736655554042No Hit
GTTATGCATGAGTCGGATAATCTTTCGTGAATAGTCGTTTAATGTTATGATAACTCGTTAAAGTTATCGTTTATG2740.16978770340442936No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2550.1580141035333193Clontech Universal Primer Mix Long (96% over 26bp)
TACCTACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAA2480.1536764614755419No Hit
TCTCTGGACTGGTGCTCCTTTCTTCGGCTGGAACAGATATGTGCCAGAAGGAAACATGACCGCTTGCGGAACTGA2440.15119780887109768No Hit
CTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTGTTCTATCGACT2360.14624050366220923No Hit
GTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAAAGGGACACGCAAATTCTCAATAGCCTGTACCGAATCCG2340.14500117735998713No Hit
GTCCTAGAATGTTAGGATGGAATGTAGCACCCGAAGATATGAAACAAATTCCCGAGCATTGGCTCAACTATGAGG2300.1425225247555429No Hit
ATATTTAGGATGGCTGATACCGTAAACGATTGGATTGTAGATGGCGTTGGCCTTGGCGAAGACTGAGCCCCAGAT2250.1394242089999876No Hit
AGCCTGTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGT2230.1381848826977655No Hit
GTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAA2110.13074892488443282No Hit
CTCATAGAGAAGCCAATGGGAGGTATCGGAAGAGTTTTCTTTTCTGTTTTACAATCGTATGCACCATGGAAGTCT2100.13012926173332176No Hit
GTGTACACGTGTTCGTTGAGCAAAGATGCAAATTTAAGCTTTCTCCTCAGCTGTGACAGCAGTGGCGACTGATTG2080.12888993543109967No Hit
ATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCC2020.12517195652443333No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.12393263022221121No Hit
CGTATGCACCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTG1890.11711633555998958No Hit
CTATGGGACCACCAATGGTTATTAACTGCTATCACGGAACATGGACTTTCGGCGCTGCTGCTTGCCAGCTTTATG1820.1127786935022122No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.1115393671999901No Hit
GGCTGATACCGTAAACGATTGGATTGTAGATGGCGTTGGCCTTGGCGAAGACTGAGCCCCAGATGGTGGCGAGTG1780.11030004089776796No Hit
CACCATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAG1700.10534273568887952No Hit
TGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATG1680.10410340938665742No Hit
GTTGTGTATGGGTTAAAGGTTAATGACATCTCGGTAACGAGAAGCTCTCATAGAGAAGCCAATGGGAGGTATCGG1640.10162475678221318No Hit
GGGCTATACAACCCCTTGGGCTGTTGCTCCAGCCATGGAACTCTGGGGACGATTTGTTCCGGAAGGATTCCTCAC1640.10162475678221318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)