FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263136
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT12280.4666788276784629No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10120.3845919980542381Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG9620.3655904171227046Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTCAGACTCGCTTTCGCTACGGCTTCCCCACACGGGTTAACCTCGCCACGTATCACTAACTCGCAGGCTCATTC8750.33252766630183633No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA6990.26564210142283834No Hit
TTCTAAGGGCGCATGGTGGATGCCTTGGCAACAGGAGCCGATGAAGGACGTGGGAATCTGCGATAAGCCTGGTGG6900.2622218168551624No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAACG6510.24740058372856621No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATA6100.23181928736470875No Hit
CACATAGACCGGGTGGAGGGAACGTGGGGAAGTGAAACATCTCAGTACCCACAGGAAGAGAAAACAAAAGTGATT6060.23029916089018607No Hit
GTACTGATTCACTATCGGTCAACAGGGAGTATTCAGGCTTACCAGGTGGTCCTGGCAGATTCACACGGGATTTCT6040.2295390976529247No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT5690.21623799100085128Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCCACCGGGTGCTGGAAGG5470.20787729539097655No Hit
GGTCTAGGACACGCGACTCAATCGCCCTATTCAGACTCGCTTTCGCTACGGCTTCCCCACACGGGTTAACCTCGC5300.20141675787425517No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAAGCACGTGAAATTGTTAAAAGGGAAG5260.19989663139973246No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGA5240.19913656816247113No Hit
GCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTA5130.19495622035753377No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGAT4860.18469536665450564No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTA4850.18431533503587497No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGG4690.17823482913778427No Hit
GGGCTAAACCGTATGCGTGTGATACCCGGCAGGGGTTGCGTGTGCGGTGTTGTGGGCCCTCTCTTGTCATGTCTG4630.17595463942600026No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGTCC4550.17291438647695487No Hit
GTGGATGCCTTGGCAACAGGAGCCGATGAAGGACGTGGGAATCTGCGATAAGCCTGGTGGAGTCGATAACCGGAC4520.1717742916210629No Hit
CATCAGGATCGGTCGATGATGCACCTTGCGGTTCTCACCTCCGTTCACTTTCATTACGCGTAGGGGTTTGACACC4400.16721391219749485No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG4350.1653137541043415No Hit
GCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCA4060.15429283716405204No Hit
GTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT4010.15239267907089868No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4000.15201264745226803Clontech Universal Primer Mix Long (96% over 26bp)
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA4000.15201264745226803No Hit
GGTTAAGGTTGTTTCAACCCCAAGGCCTCTAATCATTCGCTTTACCTCATAAAACTGAAAACGTTACTGCTATCC3930.14935242612185334No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT3840.1459321415541773No Hit
TCCCCACACGGGTTAACCTCGCCACGTATCACTAACTCGCAGGCTCATTCTTCAAAAGGCACGCTGTCACCCCTA3840.1459321415541773No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT3810.1447920466982853No Hit
GTTTAGCCCCATCCGCTTTCGCTCGCCACTACTCACGGAATCACTTTTGTTTTCTCTTCCTGTGGGTACTGAGAT3760.14289188860513194No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACCAATACCTCGCCG3700.1406116988933479No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3670.13947160403745593Clontech Universal Primer Mix Long (96% over 26bp)
GCTCTAGTAACGGCGAGTGAAGCAGCAATAGCTCAAATTTGAAATCTGGCGTCTTCGACGTCCGAGTTGTAATTT3660.13909157241882525No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG3630.13795147756293324Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC3610.1371914143256719Clontech Universal Primer Mix Long (96% over 26bp)
GTTATAGCCTCTTGTGATGCAGCGAGCGCCGGGCGAGGTCCGCGCTTCGGCTAGGATGCTGGCGTAATGGTCGTA3600.1368113827070412No Hit
GTGATAGACAGTTAAGAAGAAAAGAGAACTCTTCCCGGGGCCCCCGCCGACGTCTCCACCTTCAGTTGCGTTGCC3590.13643135108841056No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC3590.13643135108841056Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTAGATCACTCCGCTTCGGGTCTAGGACACGCGACTCAATCGCCCTATTCAGACTCGCTTTCGCTAC3560.13529125623251856No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC3510.1333910981393652No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACCAA3470.13187097166484252No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCG3440.1307308768089505No Hit
GTCGTAATGTGTTCCGCCGCTGTTGAGGGGTCGCCCGAGTAGCACGGGGCCCGAGAAATCCCGTGTGAATCTGCC3430.13035084519031984No Hit
GAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGTCGAGAGCGCACCGGACAGCACAA3380.1284506870971665No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAAT3360.12769062385990515No Hit
CTACCAATACCTCGCCGCTAGGGTAGAAACGATGCCCTAGCGAGTAGGCAGGCGTGGAGGCCCGTGACGAAGCCT3350.12731059224127447No Hit
TGCCAAGAGCGTCCGCCACCGGGTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCT3340.1269305606226438No Hit
AATCAGTACCGTGAGGGAATGGTGAAAAGTACCCCGGGAGGGGAGTGAAATAGTACCTGAAACCATGTGCCTACA3320.12617049738538247No Hit
GTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTACTACCAATACCTCGC3250.12351027605496778No Hit
TCTCAGTACCCACAGGAAGAGAAAACAAAAGTGATTCCGTGAGTAGTGGCGAGCGAAAGCGGATGGGGCTAAACC3230.12275021281770643No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCC3200.12161011796181442No Hit
GTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTACTCATAACCG3190.12123008634318375No Hit
GAGTAGCACGGGGCCCGAGAAATCCCGTGTGAATCTGCCAGGACCACCTGGTAAGCCTGAATACTCCCTGTTGAC3190.12123008634318375No Hit
GCCCTATTCAGACTCGCTTTCGCTACGGCTTCCCCACACGGGTTAACCTCGCCACGTATCACTAACTCGCAGGCT3180.12085005472455307No Hit
TTCCCAAACAACTCGACTCGTCGAAGGAGCTACGTATAGAGCGCCTTTCCGACCGCATACGGGATTCTCACCCTC3150.11970995986866108No Hit
GGTTAACCTCGCCACGTATCACTAACTCGCAGGCTCATTCTTCAAAAGGCACGCTGTCACCCCTACAAGGAGGCT3130.11894989663139974No Hit
GTTGAGGGGTCGCCCGAGTAGCACGGGGCCCGAGAAATCCCGTGTGAATCTGCCAGGACCACCTGGTAAGCCTGA3110.11818983339413838No Hit
AGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAAC3090.11742977015687704No Hit
GCCTTGGGGGTGACCCCGGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAAC3080.11704973853824638No Hit
GTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCCACCGGGTGCTGGAAGGCGCCGGCCGTG3050.11590964368235437No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC3040.11552961206372371No Hit
GTCCTGGCAGATTCACACGGGATTTCTCGGGCCCCGTGCTACTCGGGCGACCCCTCAACAGCGGCGGAACACATT2980.11324942235193967No Hit
ATGATGCACCTTGCGGTTCTCACCTCCGTTCACTTTCATTACGCGTAGGGGTTTGACACCCGAACACTCGCATAG2980.11324942235193967No Hit
CGTCAGAACCGCTGCGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCTTTCG2970.112869390733309No Hit
ACCCGGGGTCACCCCCAAGGCTTCGTCACGGGCCTCCACGCCTGCCTACTCGCTAGGGCATCGTTTCTACCCTAG2940.111729295877417No Hit
CTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAA2940.111729295877417No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCC2900.11020916940289432No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCCACCGGG2900.11020916940289432No Hit
GTTTCAGGTACTATTTCACTCCCCTCCCGGGGTACTTTTCACCATTCCCTCACGGTACTGATTCACTATCGGTCA2890.10982913778426365No Hit
CTACAAGGAGGCTCCAACGGTTTGTAGGCACATGGTTTCAGGTACTATTTCACTCCCCTCCCGGGGTACTTTTCA2850.10830901130974098No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2710.1029885686489116Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT2700.10260853703028092Clontech Universal Primer Mix Long (96% over 26bp)
GCCGTAGAGGGTGAGAGCCCCGTAGTCGTAATGTGTTCCGCCGCTGTTGAGGGGTCGCCCGAGTAGCACGGGGCC2700.10260853703028092No Hit
GCACCGGACAGCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGA2690.10222850541165024No Hit
GTCTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCTACGCGTAATGAAAGTGAACGGAGGTGAGAACCGCAAG2690.10222850541165024No Hit
GTGTGGACGCTGGGCCGTAGAGGGTGAGAGCCCCGTAGTCGTAATGTGTTCCGCCGCTGTTGAGGGGTCGCCCGA2690.10222850541165024No Hit
CCCCTACAAGGAGGCTCCAACGGTTTGTAGGCACATGGTTTCAGGTACTATTTCACTCCCCTCCCGGGGTACTTT2680.10184847379301958No Hit
CTCTATGACGTCCTGTTCCAAGGAACTTAGGTCGGTGGCCACTCAGAAGCATCCTCTACAAATTACAACTCGGAC2650.10070837893712759No Hit
GTCATAGAGGGTGAGAATCCCGTATGCGGTCGGAAAGGCGCTCTATACGTAGCTCCTTCGACGAGTCGAGTTGTT2640.10032834731849691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)