FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002151461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1318284
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG63370.48070066844473575Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG60070.4556681261397392Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50170.3805704992247497No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT35540.269592895005932Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT29290.22218277700404462No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26300.1995017765519418No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG22360.169614438163552Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21630.16407693638093157No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20230.15345706994850883Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC20230.15345706994850883Clontech Universal Primer Mix Long (96% over 26bp)
TGGTACGACCGTACCGCAAACCGACACTGGTGGGCGGGATGAGTATTCTCAGGCGCTTGAGAGAACCCAGGAGAA18480.14018223690798037No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTT18140.1376031264886777No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCGATTCTGTGCCAC17870.13555500939099616No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA17870.13555500939099616No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG17510.13282418659408748Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTCAAGGGTGTGGAACGAGCGAGCTTGCTCGCGAAGCAACAGGCAGTGGTTCCAGGAAAAGCCTCTAAGCTTCA17030.12918308953154253No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT16610.1259971296018157No Hit
CTACAAAGGCATACCTTCTCCCGAAGTTACGGTATCAATTTGCCGAGTTCCTTCTCCTGGGTTCTCTCAAGCGCC16410.1244800058257553No Hit
CCTTTGTAGCGTGTTAAAGCGCGAAGAGGTGGCACAGAATCGGTGGCTGCGACTGTTTAATAAAAACACAGCACT15960.12106647732961942No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG15800.1198527783087711Clontech Universal Primer Mix Long (96% over 26bp)
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT15180.11514969460298388No Hit
GTGCTGTGTTTTTATTAAACAGTCGCAGCCACCGATTCTGTGCCACCTCTTCGCGCTTTAACACGCTACAAAGGC14890.11294986512769632No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC14790.11219130323966611Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG14450.10961219282036344No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTGCACCCCATCACTTAACCTT13640.10346784152731885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)