FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002151470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203182
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48562.3899754899548187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30341.4932425116398105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24631.2122136803457No Hit
CTTATACACATCTCCGAGCCCACGAGACTGAATTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA20531.0104241517457255RNA PCR Primer, Index 21 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16530.8135563189652627No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.37601756061068403No Hit
TTATACACATCTCCGAGCCCACGAGACTGAATTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA7640.37601756061068403RNA PCR Primer, Index 35 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTGAATTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA7450.366666338553612RNA PCR Primer, Index 21 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.30809815830142434No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.2455926213936274No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4730.23279621226289732No Hit
TATACACATCTCCGAGCCCACGAGACTGAATTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA3370.16586114911753994RNA PCR Primer, Index 21 (96% over 28bp)
CAGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.15749426622437027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2450.1205815475780335No Hit
CAGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2140.10532429053754762No Hit
ACACATCTCCGAGCCCACGAGACTGAATTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2070.10187910346388952RNA PCR Primer, Index 21 (96% over 28bp)
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.10187910346388952No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.1008947642999872No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)