FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002151476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205921
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51222.487361658111606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31501.5297128510448181No Hit
CTTATACACATCTCCGAGCCCACGAGACTGAATTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA28081.3636297415028094RNA PCR Primer, Index 21 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22301.0829395739142682No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15620.7585433248673035No Hit
ATACACATCTCCGAGCCCACGAGACTGAATTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA9970.48416625793386786RNA PCR Primer, Index 21 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTGAATTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA8810.42783397516523325RNA PCR Primer, Index 35 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6680.3243962490469646No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.29574448453533153No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5130.24912466431301325No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4440.215616668528222No Hit
TATACACATCTCCGAGCCCACGAGACTGAATTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA3200.1553994007410609RNA PCR Primer, Index 21 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTGAATTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2890.14034508379427063RNA PCR Primer, Index 21 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2610.1267476362294278No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.11703517368311148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2190.10635146488216356No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.10198085673632121No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)