FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337940
Sequences flagged as poor quality0
Sequence length75
%GC24

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84402.497484760608392No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53121.5718766644966562No Hit
CTTATACACATCTCCGAGCCCACGAGACTGAATTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA46081.3635556607681838RNA PCR Primer, Index 21 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28270.8365390305971474No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24250.717583002899923No Hit
TTATACACATCTCCGAGCCCACGAGACTGAATTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA20740.6137184115523465RNA PCR Primer, Index 35 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTGAATTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA16960.5018642362549566RNA PCR Primer, Index 21 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13480.39888737645735933No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11540.34148073622536546No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10120.29946144285967924No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.25566668639403445No Hit
TATACACATCTCCGAGCCCACGAGACTGAATTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA7560.22370835059478014RNA PCR Primer, Index 21 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT4500.13315973249689295No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG3920.11599692253062674No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3660.10830324909747292No Hit
ACACATCTCCGAGCCCACGAGACTGAATTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA3550.10504823341421556RNA PCR Primer, Index 21 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)