FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371964
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99722.680904603671323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60611.6294587648267036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46451.2487767633426892No Hit
CTTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA42601.145272123108688Illumina PCR Primer Index 11 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26440.7108214773472702No Hit
TTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA19890.5347291673387747RNA PCR Primer, Index 45 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA15000.4032648320805239Illumina PCR Primer Index 11 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9690.2605090815240184No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7610.20458969147551914No Hit
TATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA6740.18120033121484877Illumina PCR Primer Index 11 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.1634566786033057No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT5030.13522814035766903No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4940.13280855136518588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT4810.12931358948715468No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.12205482250970524No Hit
ACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA4500.12097944962415719Illumina PCR Primer Index 11 (96% over 28bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4320.11614027163919088Clontech SMART CDS Primer II A (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)