FastQCFastQC Report
Wed 8 May 2024
EGAF00002151508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804950
Total Bases60.3 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174822.171811913783465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93861.1660351574631964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71560.888999316727747No Hit
CTTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGT40110.49829181936766265No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30020.3729424187837754No Hit
ATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTT24910.3094602149201814Illumina PCR Primer Index 11 (95% over 21bp)
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG19000.2360395055593515No Hit
TTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTC16280.2022485868687496RNA PCR Primer, Index 45 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15870.19715510280141624No Hit
ACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCT11300.14038139014845644Illumina PCR Primer Index 11 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9270.11516243244922046No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT9120.11329896266848871No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9060.11255357475619604Clontech SMART CDS Primer II A (100% over 21bp)
TATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCT8200.1018696813466675No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC8060.10013044288465123No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)