FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002151508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804950
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59220.7356978694328841No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34590.42971613143673526No Hit
CTTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA27050.3360457171252873Illumina PCR Primer Index 11 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22770.28287471271507547No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA15810.19640971488912354No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15130.1879619852164731No Hit
ATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA10610.1318094291570905Illumina PCR Primer Index 11 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACATTCCTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA9440.11727436486738307RNA PCR Primer, Index 45 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)