FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002151510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204407
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51492.51899396791695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31721.5518059557647244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24711.2088627101811582No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14300.6995846521890151No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA12010.5875532638314734Illumina PCR Primer Index 6 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7150.34979232609450756No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5430.2656464798172274No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.25830817926979016No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.24118547799243667No Hit
ATACACATCTCCGAGCCCACGAGACTCTGCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA3710.18150063353994725Illumina PCR Primer Index 6 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTCTGCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA2900.14187381058378626Illumina PCR Primer Index 6 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2610.12768642952540762No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2350.11496670857651646No Hit
CAGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.11447748854002064No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)