FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317496
Sequences flagged as poor quality0
Sequence length75
%GC23

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71252.2441227606017087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44691.4075767883689871No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA29600.932295210018394Illumina PCR Primer Index 6 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29510.9294605286365812No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19610.617645576637186No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8130.25606621815707914No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7830.24661728021770352No Hit
ATACACATCTCCGAGCCCACGAGACTCTGCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA7820.24630231561972435Illumina PCR Primer Index 6 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7400.2330738025045985No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5650.17795499785824073No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4830.1521279008239474Illumina PCR Primer Index 6 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3740.11779675964421599No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT3390.10677299871494443No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)