FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258904
Sequences flagged as poor quality0
Sequence length75
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56982.2008157463770357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33501.2939158916046103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26401.0196829712943793No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA21250.8207675431820289Illumina PCR Primer Index 6 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15680.6056298859809042No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7060.27268794611129993No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6490.25067206377653495No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.22865618144176994No Hit
ATACACATCTCCGAGCCCACGAGACTCTGCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5600.2162963878503229Illumina PCR Primer Index 6 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTCTGCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4710.18192071192411088Illumina PCR Primer Index 6 (96% over 28bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4680.1807619812749127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT3120.12050798751660848No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG3010.11625930846954856No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2920.1127831165219541No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)