FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002151520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215976
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56372.6100122235803975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34541.5992517687150425No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA24851.1505908063858947Illumina PCR Primer Index 6 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22471.0403933770418936No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15950.7385079823684113No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7430.3440197058932474No Hit
ATACACATCTCCGAGCCCACGAGACTCTGCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA6760.31299774048968404Illumina PCR Primer Index 6 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6440.298181279401415No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.2801237174500871No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.2268770604141201No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4190.1940030373745231Illumina PCR Primer Index 6 (96% over 28bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG3040.14075638033855614No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2890.13381116420343003No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2730.12640293365929547No Hit
ACACATCTCCGAGCCCACGAGACTCTGCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2390.11066044375300958Illumina PCR Primer Index 6 (96% over 28bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.10186316998184984No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.10093714116383302No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)