FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388510
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG56291.4488687549870016Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG40111.0324058582790663Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCC34660.8921263287946256No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT23160.596123651900852Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCA21210.5459318936449512No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG16700.42984736557617564Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA15880.4087410877454891Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC14200.3654989575557901Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTC12870.33126560448894493No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10910.28081645260096266Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCG10510.27052070731770095No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT9340.24040565236416053Clontech Universal Primer Mix Long (96% over 26bp)
TATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTTCTTTTCTG9280.23886129057167127No Hit
TACCTACATTGATCTATCGACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAA8380.21569586368433247No Hit
ATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCGACT8280.21312192736351704No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG8280.21312192736351704Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC7980.20540011840107075Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCT7730.1989652775990322No Hit
GTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTC7460.19201564953283057No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC7420.19098607500450437Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.1806903297212427No Hit
CTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCA6950.1788885742966719No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG6560.16885022264549174Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6410.1649893181642686Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT6330.16293016910761626Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6260.16112841368304548Clontech Universal Primer Mix Long (96% over 26bp)
GTATAAGGTATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGG5860.1508326683997838No Hit
CAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGC5720.1472291575506422No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTA5630.14491261486190832No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC5520.14208128490901137Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGCAG5450.14027952948444056Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAGGAGGTATCGGAAGAGTTTTCTTTTCTGTTTTA5190.13358729505032044No Hit
GTTGTACTCACCATGGAAGTCTTTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTG5050.12998378420117887No Hit
ATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGGCCCTT4990.1284394224086896No Hit
GTATAGGATACACGTTGATGTCTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACGGACCTGTT4980.12818202877660806No Hit
GGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAG4930.12689506061620037No Hit
GGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATT4930.12689506061620037No Hit
GGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGG4930.12689506061620037No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC4900.12612287971995573Clontech Universal Primer Mix Long (96% over 26bp)
GCCCAGGACATCTAAGGGCATCACGGACCTGTTATTGCTCAATCTCATTTTGCTAAACACAAATTGTCCTATTAA4860.12509330519162956No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG4830.12432112429538493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATACGTTTGTATAAGGTATCTTGTATATCTATACAATCTCGGTAACGAGAAACTTTCATGAAGAAGCCAATAG4810.12380633703122186No Hit
TCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAA4710.12123240071040642No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC4570.11762888986126484Clontech Universal Primer Mix Long (96% over 26bp)
AGTCTGTACCGAATCCGCAGCAGGTCTCCAAGGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGT4540.1168567089650202No Hit
TTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATATTCTCTTATTGGAC4430.11402537901212324No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGA4410.11351059174796016No Hit
GTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAAC4330.1114514426913078No Hit
CTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGA4150.10681835731384005No Hit
CACCATGGAAGTCTTTCATAGAGAGATATGGTGTAATAAATTGGTAGAGCATGGCATTAAATTGCTGTGTCGATA3980.10244266556845383No Hit
TACAAAGACCGAAAGGGAATCCGGTTACAATTCCGGAACCTGTTGAGTATACGTTTGTATAAGGTATCTTGTATA3960.10192787830429076No Hit
GTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCG3910.10064091014388304No Hit
GTACATGGGAATTCCAGGTTCATGTGAACAGTTTCAGTCCACAATCCCAACTAAGAAGACGTTTTAGTGATTTCC3890.10012612287971995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)