FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002151542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126033
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29052.304951877682829No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18731.486118714939738No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA18461.4646957542865757Illumina PCR Primer Index 1 (96% over 31bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12500.9918037339426975No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8750.6942626137598883No Hit
ATACACATCTCCGAGCCCACGAGACTCATCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5600.4443280728063285Illumina PCR Primer Index 1 (96% over 31bp)
TTATACACATCTCCGAGCCCACGAGACTCATCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4520.3586362301936794Illumina PCR Primer Index 1 (96% over 31bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4340.3443542564249046No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.2634230717351805No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.26183618576087214No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.19598041782707704No Hit
ACACATCTCCGAGCCCACGAGACTCATCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA1950.15472138249506082Illumina PCR Primer Index 1 (96% over 31bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG1760.13964596573913182No Hit
TATACACATCTCCGAGCCCACGAGACTCATCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA1720.1364721937905152Illumina PCR Primer Index 1 (96% over 31bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT1650.13091809288043607No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT1580.12536399197035697No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.10156070235573222No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT1280.10156070235573222No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)