FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342324
Sequences flagged as poor quality0
Sequence length20-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249567.290169546978885No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13020.3803414309250885No Hit
ATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGCATCATCACAAACATTT10620.31023241139972657No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTT9030.2637851859641743No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTT8760.25589792126757105No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTT8680.253560953950059No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT8570.2503476238884799No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTT8570.2503476238884799No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8180.2389549082156086Clontech Universal Primer Mix Long (96% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTT8080.23603369906871852No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8020.23428097358058447No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA7970.23282036900713943No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTT7450.21763008144331103No Hit
ATGGGAAGCAGTGGTATCAACGC7180.2097428167467078No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC7060.20623736577043972No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT6730.19659737558570242No Hit
AAGGGGAGCCCAATGGGGTTGCAAAGAATCGGTGGCTGCGACTGTTTATT6690.1954288919269464No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT6650.19426040826819038No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA6050.1767331533868499No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT5910.17264346058120378No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTT5670.16563255862866758No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCGACTTTCGTCTTTGC5470.15979014033488742No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG5410.15803741484675338No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGT5350.15628468935861933No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5240.15307135929704024No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC5110.14927378740608313No Hit
ACCATACACTGTCCCCAACCCGGATCACGGGCCAAGGTTAGAACCTCAAA5010.14635257825919304No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4730.13817319264790082No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG4610.1346677416716327No Hit
CCCTATACGTCGACTTTCGTCTTTGCAGAGTGCTGTGTTTTTAATAAACA4540.13262289526880966No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT4540.13262289526880966No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC4450.1299938070366086No Hit
ATCCTACACAGATCTGTTCAAAGTCCAATGTAAAGCTACAGTAAAGGTTC4430.12940956520723057No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT4420.12911744429254157No Hit
TAGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCC4380.12794896063378552No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTA4190.1223986632546944No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG4190.1223986632546944Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT4100.11976957502249332No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTT4040.11801684953435926No Hit
ACACAGCACTCTGCAAAGACGAAAGTCGACGTATAGGGTGTGACGCCTGC3970.11597200313153622No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGG3960.11567988221684719No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTT3930.11480351947278017No Hit
GTGTAGGATAGGTGGGAGGCTTTGAAACCTGGTCGCTAGATCAGGTGGAG3920.11451139855809117No Hit
CCATACACTGTCCCCAACCCGGATCACGGGCCAAGGTTAGAACCTCAAAC3920.11451139855809117No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAG3900.11392715672871315No Hit
ATGGGGGGTCAAGTGACTAAGTGCATGTGGTGGATGCCTTGGCGATTACA3870.11305079398464613No Hit
CCCTTGTACAGGTTCACCTACTAAGGGCACACCTTCTCCCGAAGTTACGG3750.10954534300837804No Hit
GTGCTGTGTTTTTAATAAACAGTCGCAGCCACCGATTCTTTGCAACCCCA3720.108668980264311No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTT3700.10808473843493299No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3660.10691625477617696No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3520.10282656197053083No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA3510.10253444105584184No Hit
ATTCTAAGGCGCTTGAGAGAACTCTGGAGAAGGAACTCGGCAAATTGACA3440.10048959465301878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)