FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342324
Sequences flagged as poor quality0
Sequence length20-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191485.593531274465127No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32480.9488087309098981No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18430.5383788457718419No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16800.4907631366775336No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15140.44227106483915823No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10950.31987240158446384No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10360.3026372676178124No Hit
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9140.2669985160257534No Hit
ATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGCATCATCACAAACATTT8390.24508944742407776No Hit
ATGGGAAGCAGTGGTATCAACGC6920.20214767296479358No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6880.20097918930603756Clontech Universal Primer Mix Long (96% over 26bp)
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA6540.1910470782066113No Hit
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6110.17848587887498393No Hit
AAGGGGAGCCCAATGGGGTTGCAAAGAATCGGTGGCTGCGACTGTTTATT5580.1630034703964665No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT5570.1627113494817775No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT5490.16037438216426542No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC4970.14518409460043702No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA4490.13116229069536461No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCGACTTTCGTCTTTGC4460.13028592795129762No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGT4450.1299938070366086No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG4250.12415138874282844No Hit
ACCATACACTGTCCCCAACCCGGATCACGGGCCAAGGTTAGAACCTCAAA4190.1223986632546944No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT4130.12064593776656034No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG3840.11217443124057909No Hit
TAGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCC3660.10691625477617696No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT3590.1048714083733539No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT3530.10311868288521986No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC3510.10253444105584184No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAG3460.10107383648239679No Hit
ATCCTACACAGATCTGTTCAAAGTCCAATGTAAAGCTACAGTAAAGGTTC3430.10019747373832977No Hit
ATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3430.10019747373832977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)