FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346758
Sequences flagged as poor quality0
Sequence length20-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257507.425928168924726No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13030.37576638462558903No Hit
ATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGCATCATCACAAACATTT11180.32241505603331433No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTT9630.2777152942397868No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT8590.24772319600412968No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTT8550.24656965376429674No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTT8250.23791808696554945No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTT8060.23243876132634286No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA8020.23128521908650992No Hit
ATGGGAAGCAGTGGTATCAACGC7950.22926652016680224No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTT7790.22465235120747032No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7490.21600078440872308Clontech Universal Primer Mix Long (96% over 26bp)
AAGGGGAGCCCAATGGGGTTGCAAAGAATCGGTGGCTGCGACTGTTTATT7400.2134053143690989No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTT7380.21282854324918243No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC7190.20734921760997582No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT6630.1911996262523143No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT6490.1871622284128989No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6430.18543191505314946No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA6290.1813945172137341No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCGACTTTCGTCTTTGC5970.17216617929507033No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGT5830.16812878145565494No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG5760.16611008253594725No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTT5750.165821696975989No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC5680.16380299805628132No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT5390.15543981681749233No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT5350.15428627457765937No Hit
ACCATACACTGTCCCCAACCCGGATCACGGGCCAAGGTTAGAACCTCAAA4920.14188569549945496No Hit
TAGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCC4850.13986699657974724No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG4720.13611798430029012No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT4590.13236897202083298No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG4570.1317922009009165Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC4470.12890834530133408No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4450.12833157418141758No Hit
ATCCTACACAGATCTGTTCAAAGTCCAATGTAAAGCTACAGTAAAGGTTC4420.12746641750154286No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTA4410.1271780319415846No Hit
CCCTATACGTCGACTTTCGTCTTTGCAGAGTGCTGTGTTTTTAATAAACA4290.12371740522208571No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGG4260.122852248542211No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAG4260.122852248542211No Hit
ATTCTAAGGCGCTTGAGAGAACTCTGGAGAAGGAACTCGGCAAATTGACA4150.11968000738267033No Hit
CCATACACTGTCCCCAACCCGGATCACGGGCCAAGGTTAGAACCTCAAAC4140.1193916218227121No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT4060.11708453734304616No Hit
GTGTAGGATAGGTGGGAGGCTTTGAAACCTGGTCGCTAGATCAGGTGGAG3900.11247036838371428No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTT3890.11218198282375606No Hit
CCCTTGTACAGGTTCACCTACTAAGGGCACACCTTCTCCCGAAGTTACGG3890.11218198282375606No Hit
GTGCTGTGTTTTTAATAAACAGTCGCAGCCACCGATTCTTTGCAACCCCA3830.1104516694640066No Hit
ACACAGCACTCTGCAAAGACGAAAGTCGACGTATAGGGTGTGACGCCTGC3810.1098748983440901No Hit
ATGGGGGGTCAAGTGACTAAGTGCATGTGGTGGATGCCTTGGCGATTACA3700.10670265718454945No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3700.10670265718454945No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTT3660.10554911494471649No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3620.10439557270488353No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA3510.10122333154534285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)