FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427453
Sequences flagged as poor quality0
Sequence length20-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130703.0576461037821705No Hit
ATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGC27450.6421758649488949No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG21000.4912820824745644No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC18360.42952090639204776No Hit
ATGGGCAGAATTCACAATCCGTCTTCACGCTTTGACACCATCGGATTGCT15330.35863592020643204No Hit
GTGCTGGGCTGTTCGAAAGGCGGGCGAATCAGGAGTCTCGGGCTTTCGGG10410.2435355465981055No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT10110.23651723113418316No Hit
GTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGG10000.23394384879741165No Hit
CTCCTAGACTTCATAAGTTCTATGTTTGGTCGTTAATTAGTGGCCAGATG9870.23090257876304526No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA9480.22177876865994622No Hit
GCCCAGCACACTCGACCTATAGAGCCAATCCTTTTCCCTAAGTTACGGAT8140.19043029292109306No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT7910.1850495843987526No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA7900.1848156405499552No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC7640.17873310048122248No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG7580.177329437388438No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7370.17241661656369237No Hit
ATGGGTAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGA7170.16773773958774416No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7100.16610013264616227No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATC6900.16142125567021404No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA6590.15416899635749426No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA5550.12983883608256347No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC5460.12773334144338674No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGG5280.12352235216503335No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAG5150.120481082130667No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT5150.120481082130667No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG4960.11603614900351616No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT4840.11322882281794724No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA4800.11229304742275759No Hit
GTCTTTGGCCGATGGGAACAAAGTAGCGTAAGGGAATTCGGCAAAATAGA4750.11112332817877052No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC4700.10995360893478348No Hit
ATGGGTGTTCTTCAGTGCGATAGTCAAGATGGTTCGTATTCCATCGCCAG4560.10667839505161972No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC4530.10597656350522747No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG4520.10574261965643006No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4300.100595854982887No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4290.1003619111340896No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT4280.10012796728529219No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)