FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228161
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCACGGAAGCAGCTCGAGCAGTCCGCCGACAG19250.8437024732535359No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGG18580.814337244314322No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG16230.7113397995275267Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG15610.6841660055837764Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTGAGTTGACTGTTCGACGCCCGGGGAAGGCCCCCGAAGGGACCGTTCCCAGTCCGTCCCCCGGCCGGCACG11280.49438773497661737No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTC10730.4702819500265164No Hit
GCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCT9550.4185640841335723No Hit
GCGTTAACATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT8250.36158677425151536No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGG8200.35939533925605166No Hit
ACCTATACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGGCGCAGCGGTCGCTGCAAAACCTGTG7930.3475615902805475No Hit
GTCCCGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTTCCGTGGCGAGAGCGGGTCGCCGCGTGCCGGCCG7760.34011071129597087No Hit
CCCCTTGTCCGTACCAGTTCTGAGTTGACTGTTCGACGCCCGGGGAAGGCCCCCGAAGGGACCGTTCCCAGTCCG7750.33967242429687805No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC7490.32827696232046666Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT7210.3160049263458698Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATAGGTCACGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT7170.3142517783494988No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAACATCCGCAGGGACCATCGCAATGCTTTGTTTTA7030.30811576036220034No Hit
CCCCAGGTCAGGCGGGGCTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCC6870.3011031683767164No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAA6630.2905842803984905No Hit
GTCCGTACCAGTTCTGAGTTGACTGTTCGACGCCCGGGGAAGGCCCCCGAAGGGACCGTTCCCAGTCCGTCCCCC6050.2651636344511113No Hit
GTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCATTTTGCCGAC5880.2577127554665346No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTTAACATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAA5860.25683618146834913No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAGTATTTAGCCTTGGACGGAATTTACCGCCCACTTGGGGCTGCATTCC5450.2388664145055465No Hit
GACTGGGAACGGTCCCTTCGGGGGCCTTCCCCGGGCGTCGAACAGTCAACTCAGAACTGGTACGGACAAGGGGAA5410.23711326650917552No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAACA5220.22878581352641336No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5140.2252795175336714Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCAGAAATCACATTGCGTTAACATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGG5130.22484123053457863No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGACTGTTCGA5050.2213349345418367No Hit
GTCCGCCGACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTT4970.2178286385490947No Hit
TTCCCAAACAACCCGACTCGGCGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCT4910.21519891655453824No Hit
GCGCTGAAGCGCGTGACCTATACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGGCGCAGCGGTC4900.21476062955544548No Hit
CCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGAC4810.21081604656361078No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGACTGTTCGACGCCC4610.20205030658175588No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG4600.20161201958266312Clontech Universal Primer Mix Long (96% over 26bp)
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACT4550.19942058458719938No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT4550.19942058458719938Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAACATCCGCAGGGACCATCGCAATGCTTTGTTTTAA4430.19416114059808642No Hit
GCCCTGGCTCACGCCACAGGTTTTGCAGCGACCGCTGCGCCCTCCTACTCATCGGGGCCTGGCACTTGCCCCGAC4370.19153141860352996No Hit
ACCCCAGGTCAGGCGGGGCTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTC4350.19065484460534446No Hit
AGGTAGGACGGCGCGGCTGCTTTGTTGAGCCGTGCCATGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGC4300.18846340960988073No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4290.188025122610788Clontech Universal Primer Mix Long (96% over 26bp)
CCCCCGAAGGGACCGTTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCACGGAAGCAGCTCGA4210.18451882661804603No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC4180.1832039656207678Clontech Universal Primer Mix Long (96% over 26bp)
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAA4120.18057424362621133No Hit
CCCGGGGAAGGCCCCCGAAGGGACCGTTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCACGG4070.17838280863074757No Hit
ACCGGGAACAGCCCAGCTTTAAAATCGGGCGGCTTCGTCGTCCGAATTGTAGTCTGGAGAAGCGTCCTCTGCGGC4020.17619137363528387No Hit
GAGTAGGAGGGCGCAGCGGTCGCTGCAAAACCTGTGGCGTGAGCCAGGGCGGAGCGGCCGTTGGTGCAGATCTTG3950.17312336464163464No Hit
GTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCAGCCCCTTTCCAGGGGACTTGGGCCCGGTCCGCCGCAGAGG3920.1718085036443564No Hit
GCTCTGAGGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTTCCGTG3870.16961706864889267No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGTCGGCACTCGGTCCTCCGG3750.1643576246597797No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG3720.16304276366250148Clontech Universal Primer Mix Long (96% over 25bp)
GACCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGG3680.1612896156661305No Hit
CCCTGGAATCGACTCAGTCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCGCACGTTTTGTGGTGTCCGGTGCGC3660.160413041667945No Hit
GTCGGCGGACTGCTCGAGCTGCTTCCGTGGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGGACGGACTGGGAACG3620.15865989367157401No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3550.15559188467792479Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCTACTCATCGGGGCCTGGCACTTGCCCCGACGGCCGGGTATAGGTCACGCGCTTCAGCGCCATCCATTTTCG3480.15252387568427558No Hit
AACCTAGATCCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGG3460.15164730168609009No Hit
TACCAGTTCTGAGTTGACTGTTCGACGCCCGGGGAAGGCCCCCGAAGGGACCGTTCCCAGTCCGTCCCCCGGCCG3460.15164730168609009No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3420.1498941536897191Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCGGACTTCCT3330.1459495706978844No Hit
GAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCA3330.1459495706978844No Hit
GTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCAGCCCCTTTCCAGGGGACTTGGGCCCGGTCC3300.14463470970060616No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG3280.14375813570242066Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACGACCGGGCGTGGTCGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCACAA3250.14244327470514243No Hit
ATCCTAAGAGACGGGGGAAGCCTGTCCGATAGCGCGTTTCGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTCC3240.1420049877060497No Hit
GTGCCCAACTACGCGCTAACCTAGATCCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAA3210.14069012670877146No Hit
GTATTTAGCCTTGGACGGAATTTACCGCCCACTTGGGGCTGCATTCCCAAACAACCCGACTCGGCGACAGCGCCT3200.1402518397096787No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAACATCCGCAGGGACCATCGCAATGCTTTGTTTT3170.13893697871240046No Hit
TCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCG3110.13630725671784397No Hit
TTGTTGAGCCGTGCCATGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGA3110.13630725671784397No Hit
GGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTTCCGTGGCGAGAGCGGGT3080.13499239572056573No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGTGACCTATACCCGGCCG3050.1336775347232875No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGTGACCTA3040.13323924772419476No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT2960.12973295173145277Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2960.12973295173145277Clontech Universal Primer Mix Long (96% over 26bp)
GGGCTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCCCTAGTAACGGCGA2900.1271032297368963No Hit
GTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCATTCGCGAGTTCTATCAGGTAAAGCC2890.12666494273780357No Hit
GTCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCGCACGTTTTGTGGTGTCCGGTGCGCCCCCGGCGGCCCTTGA2880.1262266557387108No Hit
CTCTCTGGCAGCCCCTTTCCAGGGGACTTGGGCCCGGTCCGCCGCAGAGGACGCTTCTCCAGACTACAATTCGGA2880.1262266557387108No Hit
GTACATGGGGCGACCCCAGGTCAGGCGGGGCTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAAC2840.12447350774233985No Hit
GTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCGAGGCCTCTAATCATTGGCTTTACCTG2810.12315864674506159No Hit
ACGTTAGGAAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCT2800.12272035974596886No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGTGACCTATACCCGGC2800.12272035974596886No Hit
AAATCACATTGCGTTAACATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTC2800.12272035974596886No Hit
GTACATGGGTGCCGACCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGAT2790.12228207274687611No Hit
GTTCGACGCCCGGGGAAGGCCCCCGAAGGGACCGTTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCT2760.12096721174959786No Hit
TCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCGAAGCTCGCGCGAAA2740.12009063775141238No Hit
CGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGG2730.11965235075231963No Hit
CTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAA2730.11965235075231963No Hit
CTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTTCG2660.11658434175867041No Hit
TCCCAGTCCCGAACCCGTCGGCTGTCGGCGGACTGCTCGAGCTGCTTCCGTGGCGAGAGCGGGTCGCCGCGTGCC2640.11570776776048493No Hit
GGGGAAGCCTGTCCGATAGCGCGTTTCGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTCCTGAACCGGGACGT2630.11526948076139218No Hit
GAGTTGACTGTTCGACGCCCGGGGAAGGCCCCCGAAGGGACCGTTCCCAGTCCGTCCCCCGGCCGGCACGCGGCG2610.11439290676320668No Hit
GAAGAGCACCGCACGTTTTGTGGTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCGAC2600.11395461976411393No Hit
GTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCGACCACGCCCGGTCGTACTCATAACCG2590.11351633276502118No Hit
GTGCTCTTCCAGCCGCTGGACCCTACCTCCGACTGAGTCGATTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGAT2590.11351633276502118No Hit
CCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGACC2570.1126397587668357No Hit
CATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTT2530.1108866107704647No Hit
ATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGG2500.10957174977318648No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC2480.108695175775001Clontech Universal Primer Mix Long (96% over 26bp)
GCTCTCGCCACGGAAGCAGCTCGAGCAGTCCGCCGACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCC2440.10694202777863No Hit
CCCCTAGTAACGGCGAGCGAACCGGGAACAGCCCAGCTTTAAAATCGGGCGGCTTCGTCGTCCGAATTGTAGTCT2390.10475059278316626No Hit
GTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCTGGAATCGACT2290.10036772279223881No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)