FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411008
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG95652.3272053098723138Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG80521.9590859545312989Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT49021.1926775147928994Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC36300.8831944876985363Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG30510.742321317346621Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG29100.7080154157583307Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC28140.6846582061663034Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA26070.6342942229834942Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT22920.5576533790096543Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG22880.5566801619433198Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20800.5060728744939271Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC20620.501693397695422Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG18000.4379476798505139Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT17860.4345414201183432Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC15590.3793113516038617Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC15340.3732287449392713Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15110.367632746807848No Hit
GTTATGGCTAGGGGGTCATTGCGACTTACCAAACCATTGCAAACTCCGAATACCGATGAGTGCGAGCTTGGGAGA10890.2649583463095609No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10770.26203869511055744Clontech Universal Primer Mix Long (96% over 26bp)
AGCCATAACAGTGCTCTACCCCCGATGGTGATACTTGAGGCACTACCTAAATAGTTTTCGGAGAGAACCAGCTAT10680.2598489567113049No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT10650.25911904391155405Clontech Universal Primer Mix Long (96% over 25bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9480.23065244472127064Clontech Universal Primer Mix Long (96% over 26bp)
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAG9200.2238399252569293No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG9060.22043366552475865Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG8880.21605418872625348Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCATACGACGGTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGCCGACTCA8870.21581088445966987No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.21094479912799752No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCAGTTTATTGCAAC8610.20948497352849582No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC8430.20510549672999065Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA8410.20461888819682342Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTACTAAGCGTACGACAAGTAGGGCGGGACACGAGAAATCCTGTCTGAAGATGGGGGGACCATCCTCCAAGG8010.19488671753347866No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC7820.19026393646838993Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG7540.18345141700404857Clontech Universal Primer Mix Long (96% over 26bp)
GTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGCCGACTCACCCTGCTCCG7510.18272150420429772No Hit
TTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCTGACTGTTGGAATAGTCAGTTTCT7110.17298933354095297No Hit
GTGAATAAGTGCACATGGTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGG7080.17225942074120212No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC7040.17128620367486766Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT6990.17006968234194955Clontech Universal Primer Mix Long (96% over 26bp)
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA6980.1698263780753659No Hit
GTGTAGACCCGAAACCAGGTGATCTACCCATGGCCAGGATGAAGGTGCGGTAACACGCCCTGGAGGTCCGAACCC6930.16860985674244783No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG6850.1666634226097789Clontech Universal Primer Mix Long (96% over 26bp)
GTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCTGACTGTTGGAATAGTCAGTTTCTGGTTCATAGA6810.1656902055434444No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA6810.1656902055434444Clontech Universal Primer Mix Long (96% over 26bp)
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT6770.16471698847710992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.16423037994394268No Hit
GTCTCGGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT6600.16058081594518842No Hit
GGGTGAGTCGGCCCCTAAGGCGAGGCAGAGATGCGTAGCTGATGGGAAGCAGGTTAATATTCCTGCACCGTCGTA6310.15352499221426347No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC6250.15206516661476177Clontech Universal Primer Mix Long (96% over 25bp)
ATACATAGGTATGCGAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCTGCAGGAAAAGAAATCAACCGAGATT6210.15109194954842728No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6150.14963212394892556Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA6080.14792899408284024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCAGTTTATTGCAACCC6030.14671247274992213No Hit
GTACTGGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATCTTCAGACAGGATTTCT5910.14379282155091871No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT5780.14062986608533168Clontech SMART CDS Primer II A (100% over 26bp)
CCTTAGAATACTCATCTCGCCCACCTGTGTCGGTTTGCGGTACGGTCTCGTATGACTGAAGCTTAGAGGCTTTTC5690.1384401276860791No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA5650.13746691061974461Clontech Universal Primer Mix Long (96% over 26bp)
ATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGGTACCGTAACTTCGGGAAAAGGTACGCC5570.1355204764870757No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT5470.1330874338212395Clontech Universal Primer Mix Long (96% over 26bp)
ATACGAGACCGTACCGCAAACCGACACAGGTGGGCGAGATGAGTATTCTAAGGCGCTTGAGAGAACTCGGGAGAA5360.1304110868888197No Hit
AACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGGAGGGGAGTGAAATAGATCCTGAAACCGTGTGCATACA5330.12968117408906885No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT5150.1253016972905637Clontech SMART CDS Primer II A (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT5110.12432848022422922Clontech Universal Primer Mix Long (96% over 25bp)
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCTT5060.12311195889131112No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC5010.12189543755839302Clontech Universal Primer Mix Long (96% over 26bp)
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGTTACGGC4910.11946239489255685No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC4910.11946239489255685Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT4810.11702935222672065Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT4780.1162994394269698Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGTAAGCCCAAGGTTTCCTGTTCAACGTTCATCGGAGCAGGGTGAGTCGGCCCCTAAGGCGAGGCAGAGATGCG4730.1150829180940517No Hit
GGTCTACACCCAGCGACTGTCGCCCTGTTCGGACTCGATTTCTCTACGGCTCCCCTATTCGGTTAACCTCGCCAC4640.11289317969479912No Hit
GACTCACCCTGCTCCGATGAACGTTGAACAGGAAACCTTGGGCTTACGGCGTGCGGGCTTTTCACCCGCATTATC4630.1126498754282155No Hit
GTGCGAGCTTGGGAGACAGACATCGGGTGCTAACGTCCGGTGTCAAGAGGGAAACAACCCAGACCGCCAGCTAAG4620.11240657116163189No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT4580.11143335409529742Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4480.10900031142946122No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC4440.10802709436312674Clontech Universal Primer Mix Long (96% over 26bp)
TAGTACCACCGTCTGAATTTCGTGTACGGGGCTATCACCCGCTATGGCGCCTATTTCCAGAGGCTTCCACTATTC4360.1060806602304578No Hit
CTCCTACCATATCCAATAAAGGATATCCGCAGCTTCGGTGACTGGCTTAGCCCCGTTACATCTTCCGCGCAGGAC4300.1046208346309561No Hit
GTATCAACGCAGAGTACATGGGGTCTTTCCGTCTAGCCGCGGGTAGATTGCATCATCACAAACATTTCAACTTCG4270.10389092183120523No Hit
GTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATAAGCTGCGGGGAGCTAGCAAACGAGC4230.10291770476487075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)