FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269971
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG28261.0467790984957643Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG23980.8882435520852239Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT17490.6478473613832597Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.47190253767997303No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11750.4352319323186565Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC11630.43078701045667867Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11470.4248604479740416Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10900.4037470691296473Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10160.376336717647451Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG7570.2804004874597642Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC7480.2770667960632809Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7370.27299228435646794No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC7100.26299121016701793Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT6930.2566942375292161Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG6820.25261972582240316Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAG6000.2222460930988884No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG5910.21891240170240509Clontech Universal Primer Mix Long (96% over 25bp)
AGCCATAACAGTGCTCTACCCCCGATGGTGATACTTGAGGCACTACCTAAATAGTTTTCGGAGAGAACCAGCTAT5470.20261435487515328No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT5180.19187246037537367Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5090.18853876897889033Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.185205077582407No Hit
GGTCTACACCCAGCGACTGTCGCCCTGTTCGGACTCGATTTCTCTACGGCTCCCCTATTCGGTTAACCTCGCCAC4840.17927851509976997No Hit
GTTATGGCTAGGGGGTCATTGCGACTTACCAAACCATTGCAAACTCCGAATACCGATGAGTGCGAGCTTGGGAGA4690.17372236277229777No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC4270.15816513625537557Clontech Universal Primer Mix Long (96% over 26bp)
GTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCCAGCTGTTGGAATAGCTGGTTTCTGGTTCATAGA4180.15483144485889225No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCAGTTTATTGCAAC3900.14445996051427745No Hit
GTACTGGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAGACAGGATTTCT3730.13816298787647563No Hit
GTGGTACTAAGCGTACGACAAGTAGGGCGGGACACGAGAAATCCTGTCTGAACATGGGGGGACCATCCTCCAAGG3710.13742216756614598No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT3620.13408847616966266No Hit
GTCTCGGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT3620.13408847616966266No Hit
TTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGTCCAGCTGTTGGAATAGCTGGTTTCT3500.1296435543076849No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC3440.12742109337669602Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.12556904260087196No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT3390.12556904260087196No Hit
TCATACGACGGTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGCCGACTCA3320.12297617151471826No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3140.1163087887217516Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG3130.11593837856658679Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGTGCGCCGTTCCTCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGAATGTGGATTCTCCACGGCAACGTAA3130.11593837856658679No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGACGTGAAAACTATTCCCTCCGGCGG3110.11519755825625715No Hit
GTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCCTTAGGGGCCGACTCACCCTGCTCCG3100.11482714810109233No Hit
ATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGGTACCGTAACTTCGGGATAAGGTACGCC3020.11186386685977383No Hit
ATACGAGACCGTACCGCAAACCGACACAGGTGGGCGAGATGAGTATTCTAAGGCGCTTGAGAGAACTCGGGAGAA3020.11186386685977383No Hit
GGGTGAGTCGGCCCCTAAGGCGAGGCAGAGATGCGTAGCTGATGGGAAGCAGGTTAATATTCCTGCACCGTCGTA2960.10964140592878494No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA2940.10890058561845532No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC2890.10704853484263124Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT2890.10704853484263124Clontech Universal Primer Mix Long (96% over 25bp)
GTTCTAAGTTGGTTGTTAATTGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCTGAACCCCGATGCTCCCGGG2720.1007515622048294No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2700.10001074189449977Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)