FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403539
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT39120.969423029744337No Hit
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGG31920.7910016132269743No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG29480.7305365776294237No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA22170.5493892783597124No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCA22160.5491414708367717No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG21460.531794944230917No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAG20910.518165530469174No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAGTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT20740.5139528025791807No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT19710.4884286277162802No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCG18980.4703386785416032No Hit
GCTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCAC18580.4604263776239719No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGG17780.4406017757887094No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTAC17340.429698244779315No Hit
CTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACC16140.39996134202642125No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA15910.39426176899878324No Hit
GAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATGCTGGCACCCTTGGCAGGCTTCGGCCGTCCGG15700.38905781101702686No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC14950.37047224679646823No Hit
GCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATGCTGGCA14700.36427705872294874No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT13890.34420464936474543No Hit
GCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCG13780.34147876661239684No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTT13610.3372660387224035No Hit
GGGTACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTG13350.33082304312594324No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTA11920.29538656734541147No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCC11360.2815093460607277No Hit
GTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATGCTGGCACCCTTGGCAGGCTTCGGCCGTCCGGCGTAC11320.2805181159689646No Hit
CGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATG11220.27804004073955674No Hit
ATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCC10870.2693667774366294No Hit
GCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGT10380.25722420881253116No Hit
CTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTT10360.2567285937666496No Hit
GGATTGGCTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGT10280.2547461335831233No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGC10280.2547461335831233No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGAC10150.25152463578489315No Hit
TATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAA10000.24780752294078146No Hit
GTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGGTGCCCACCTTG9640.2388864521149133No Hit
GCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGT9310.23070880385786752No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC8900.22054869541729547No Hit
GTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT8790.21782281266494685No Hit
GGGTTGGGTACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAG8440.20914954936201952No Hit
GCCGCAAGGTGGGCACCTTTCAGCGCTGGGATGCTGGCACCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAA8330.2064236666096709No Hit
GTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTTGGTGCGCTCCCGACGAGCCTTGA8250.20444120642614466No Hit
CTCCCGACGAGCCTTGAAAATCCGCTTGAAGAAATAGTTTTTACGCCAGGTCGTACTCATAACCGCAGCAGGTCT8230.2039455913802631No Hit
CCTTTCAGCGCTGGGATGCTGGCACCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAAC8070.19998067101321063No Hit
ATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAA7930.19651136569203967No Hit
CCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGAC7900.19576794312321733No Hit
GGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATT7890.19552013560027656No Hit
TTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTG7740.19180302275616484No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAA7600.18833371743499389No Hit
GGTGGGCACCTTTCAGCGCTGGGATGCTGGCACCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAA7590.18808590991205312No Hit
GGCTGGAAGAGCGCTGCACTTTTGCGGCGTTTGGTGCGCTCCCGACGAGCCTTGAAAATCCGCTTGAAGAAATAG7580.18783810238911233No Hit
GCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAA7490.1856078346826453No Hit
GTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAAAAACTATTTC7490.1856078346826453No Hit
TTTCAGCGCTGGGATGCTGGCACCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTG7470.18511221963676375No Hit
GCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATGCTGGCACCCTTGGCAGGCTTCGGCCG7350.18213852936147437No Hit
CCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCC7320.18139510679265203No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGG7270.1801560691779481No Hit
GTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTT7200.17842141651736265No Hit
GTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGC7190.17817360899442183No Hit
GATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCC7160.17743018642559952No Hit
CCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCT7090.17569553376501404No Hit
GAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTTGGTGCGCTCCCGACGAGCCTTGAAAATC7040.17445649615031014No Hit
TGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCA6820.16900473064561294No Hit
ACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGAT6710.16627884789326433No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGCCAGAAAGTGGTG6690.16578323284738278No Hit
AAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGACGCTTTCGG6680.165535425324442No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT6570.1628095425720934No Hit
CTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAA6550.16231392752621185No Hit
GATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCCT6470.1603314673426856No Hit
TGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCT6450.15983585229680403No Hit
GGCTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCA6020.14918012881035042No Hit
GAGTACGACCTGGCGTAAAAACTATTTCTTCAAGCGGATTTTCAAGGCTCGTCGGGAGCGCACCAAACGCCGCAA6010.14893232128740966No Hit
ATCCTAAGAGATAGGGAAACTCCGTTTTAATGTGGGCGCTTGCGCCCCACCCTCGAAAGGGAAGCCGGTTAACAT5740.14224151816800853No Hit
GGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCC5730.14199371064506777No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA5710.1414980955991862No Hit
CTTCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTT5690.14100248055330464No Hit
GGGTGCCAGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAA5540.13728536770919292No Hit
AGCCTTTACCGTGTAAAGCCCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCT5460.13530290752566668No Hit
TCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTA5440.1348072924797851No Hit
CCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCAT5340.13232921725037727No Hit
GGTTGGGTACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGC5260.13034675706685103No Hit
GCTGAAAGGTGCCCACCTTGCGGCTAGTACCCTTCTGCGCCGAAAGCGTCTCCCCAAGGCCCAACGTACCCAACC5200.12885991192920634No Hit
GGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCT5180.1283642968833248No Hit
CTTCCAGCCATTGAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCT5100.12638183669979852No Hit
TCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG5050.12514279908509462No Hit
AGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG5020.12439937651627228No Hit
GTTTTAATGTGGGCGCTTGCGCCCCACCCTCGAAAGGGAAGCCGGTTAACATTCCGGCACCTGGATGTAGATTCT5010.1241515689933315No Hit
GAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGT4900.1214256862409829No Hit
CGTCGGGAGCGCACCAAACGCCGCAAAAGTGCAGCGCTCTTCCAGCCATTGAACCCTAGCTCCGGACAAACCGAT4870.12068226367216055No Hit
TACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTG4860.12043445614921977No Hit
TCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTG4770.11820418844275275No Hit
CGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCCTTGGGGAGACGCT4720.11696515082804883No Hit
GGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATGCTGGCACCCTTGGCAGGCTTCGGCCGTCCGGCGT4710.11671734330510805No Hit
GGTACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGG4650.11523049816746338No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGC4650.11523049816746338No Hit
GTACTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGC4610.11423926807570026No Hit
GTTGGGTACGTTGGGCCTTGGGGAGACGCTTTCGGCGCAGAAGGGTACTAGCCGCAAGGTGGGCACCTTTCAGCG4530.11225680789217399No Hit
GGTTAGTCGATCCTAAGAGATAGGGAAACTCCGTTTTAATGTGGGCGCTTGCGCCCCACCCTCGAAAGGGAAGCC4460.11052215523158852No Hit
GTAAAGGCTAGCGACCACGTACGGGATTCTCACCCTCTGTGACGTCCTGTTCCAAGGAACTTGGACCGCTGCCAA4460.11052215523158852No Hit
CGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTT4380.10853969504806228No Hit
CATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAAT4350.10779627247923992No Hit
GGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTT4300.10655723486453601No Hit
GTATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGGGCTT4230.10482258220395055No Hit
GTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGG4220.10457477468100976No Hit
GTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGG4210.10432696715806898No Hit
GCGCTGGGATGCTGGCACCCTTGGCAGGCTTCGGCCGTCCGGCGTACGCTTAACAACCAACTTAGAACTGGTACG4170.10333573706630586No Hit
GGTACTAGCCGCAAGGTGGGCACCTTTCAGCGCTGGGATGCTGGCACCCTTGGCAGGCTTCGGCCGTCCGGCGTA4160.10308792954336508No Hit
AGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGGTGCCCACCTTGCGGCTAG4060.10060985431395726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)