FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414153
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG22600.5456920510053048Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20870.503920048870828Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTT15590.37643093252976556No Hit
GTAGTATTTAGCCTTACGAGATGGTCCTCGCTGATTCACACGGGATTTCACGGGCCCCGTGCTACTCGGGATACA11880.2868505117673903No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11440.27622641873896847Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG11290.27260456884291556No Hit
GTACTGGTTCACTATCGGTCACACAGTAGTATTTAGCCTTACGAGATGGTCCTCGCTGATTCACACGGGATTTCA9380.22648634683317517No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG8630.20837709735291063Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8480.20475524745685775No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA8250.19920174428290993Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC8020.19364824110896214Clontech Universal Primer Mix Long (96% over 26bp)
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG7120.17191714173264472No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACC6420.15501517555106445No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT6360.1535664355926433No Hit
GGATACAGTCACGCTCTTTTCTACTTTCGACTACAGGACTTTCACCTTCTCTGGTGCAGCTTTCAACTGCTTTGT6340.1530835222731696No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG6080.1468056491200112Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG5520.133284076174747Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5430.13111096623711527Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCACTATCGGTCACACAGTAGTATTTAGCCTTACGAGATGGTCCTCGCTGATTCACACGGGATTTCACGGGCC5240.12652328970211493No Hit
CAGTAGTATTTAGCCTTACGAGATGGTCCTCGCTGATTCACACGGGATTTCACGGGCCCCGTGCTACTCGGGATA5080.12265998314632515No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT5010.12096978652816712Clontech Universal Primer Mix Long (96% over 26bp)
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC4960.11976250322948281No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGAT4960.11976250322948281No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4910.11855521993079851Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC4580.11058715015948213Clontech Universal Primer Mix Long (96% over 26bp)
ACCCAGTAAGCATCAGTTGAATACATAGACTGAGAGCAGCGAACCCAGCGAATTGAAACATCTTAGTAGCTGGAG4540.10962132352053469No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4470.10793112690237665No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTT4450.10744821358290294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)