FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002151946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302289
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77022.5478929104267767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45141.4932729937245484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34221.1320292832355794No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCAGCCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA28510.9431371965238564Illumina PCR Primer Index 4 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21060.6966842988001548No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.2858191995077558No Hit
ATACACATCTCCGAGCCCACGAGACTCCAGCCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA7820.25869284029521417Illumina PCR Primer Index 4 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7690.25439231993225025No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7600.25141503660404446No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6110.20212445705930418No Hit
TTATACACATCTCCGAGCCCACGAGACTCCAGCCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA5900.195177462626824Illumina PCR Primer Index 4 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT3240.10718219981540843No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3140.10387410722851312No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)