FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002151978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256826
Sequences flagged as poor quality0
Sequence length75
%GC23

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78803.068225179693645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48391.8841550310326836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32611.2697312577387023No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22200.8643984643299355No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA21290.8289659146659606TruSeq Adapter, Index 3 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10080.3924836270471058No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8140.316946103587643No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7480.2912477708643206No Hit
ATACACATCTCCGAGCCCACGAGACTAAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA7000.2725580743382679TruSeq Adapter, Index 3 (96% over 28bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.2558152212003457No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA5890.22933815112177117TruSeq Adapter, Index 3 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT4020.156526208405691No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT4000.15574747105043882No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.13627903716913395No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.11681060328782912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT2670.10396143692616791No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)