FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002151988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144665
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32462.243804652127329No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19681.3603843362250716No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA19331.336190509107248Illumina PCR Primer Index 1 (96% over 31bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12020.8308851484464107No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8780.6069194345556976No Hit
ATACACATCTCCGAGCCCACGAGACTCATCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5100.35253862371686306Illumina PCR Primer Index 1 (96% over 31bp)
TTATACACATCTCCGAGCCCACGAGACTCATCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4570.315902256938444Illumina PCR Primer Index 1 (96% over 31bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.2827221511768569No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.255072063042201No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.24885079321190334No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2840.19631562575605713No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2100.14516296270694365No Hit
ACACATCTCCGAGCCCACGAGACTCATCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2000.13825044067327963Illumina PCR Primer Index 1 (96% over 31bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT1760.1216603877924861No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG1630.11267410914872292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT1500.10368783050495974No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)