FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002151990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250730
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58112.3176325130618594No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36941.4732979699278108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23510.9376620268815059No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA22370.8921947912096677Illumina PCR Primer Index 1 (96% over 31bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16150.6441191720177083No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8040.3206636621066486No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6620.26402903521716586No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6130.24448610058628806No Hit
ATACACATCTCCGAGCCCACGAGACTCATCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA6040.2408965819806166Illumina PCR Primer Index 1 (96% over 31bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5360.21377577473776574No Hit
TTATACACATCTCCGAGCCCACGAGACTCATCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4030.16073066645395445Illumina PCR Primer Index 1 (96% over 31bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3050.12164479719219878No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT3030.12084712639093846No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.10210186256132095No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)