FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002151994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002151994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207957
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47722.2947051553927014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27131.304596623340402No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19730.9487538289165549No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14690.706396033795448No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA9410.45249739128762195Illumina PCR Primer Index 1 (96% over 31bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6990.33612718013820164No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5860.28178902369239794No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.26495862125343217No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.2370682400688604No Hit
ATACACATCTCCGAGCCCACGAGACTCATCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA2640.12694932125391306Illumina PCR Primer Index 1 (96% over 31bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2410.11588934250830701No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2390.11492760522608038No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG2130.1024250205571344No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)