FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002152018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194675
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49182.526261718248363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29721.5266469757287788No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCCGGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA29481.5143187363554642RNA PCR Primer, Index 17 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22781.1701553871837678No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13980.7181199434955696No Hit
ATACACATCTCCGAGCCCACGAGACGTCCGGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA7070.36316938487222294RNA PCR Primer, Index 17 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.3457043790933607No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.3128290740978554No Hit
TTATACACATCTCCGAGCCCACGAGACGTCCGGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA5970.3066649544111981RNA PCR Primer, Index 47 (96% over 29bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.25016052395017335No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.2059843328624631No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2320.11917298060870683No Hit
ACACATCTCCGAGCCCACGAGACGTCCGGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2300.11814562732759729RNA PCR Primer, Index 17 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGTCCGGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA2260.11609092076537819RNA PCR Primer, Index 17 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2250.11557724412482341No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)