FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002152020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265922
Sequences flagged as poor quality0
Sequence length75
%GC24

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71242.6789810545949564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44381.668910432382428No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29881.123637758440445No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCCGGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA23110.8690518272275328RNA PCR Primer, Index 17 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20140.7573649415994164No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9990.37567406984002827No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8010.3012161460879506No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7550.28391784056979114No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6380.2399199765344725No Hit
ATACACATCTCCGAGCCCACGAGACGTCCGGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5870.2207414204165131RNA PCR Primer, Index 17 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGTCCGGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4480.16847045374207475RNA PCR Primer, Index 47 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3460.13011334150615594No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT3100.11657553718759638No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)