FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002152203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425437
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38840.9129436320771348No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC21960.5161751328633852No Hit
CCCCAGGGACCGTCTGCAGCCTCCTGCTCCTCGGCATGCTCTGGCTGGAC13910.3269579279658328No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT11090.2606731431445784No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT9230.2169533914539638No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC9010.21178223802819218No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA8540.20073477389131647No Hit
ATGGGACCTCCGCCAGGAACTGCAGGCCCACCTGTCTGCAACCCAGCTGA7610.17887489804600915No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC7530.17699447861845585No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA6720.15795523191447852No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG6580.15466449791626022No Hit
AACAGTCGTGGGAGTTGCTGCAGAAGCAAGCGAAAAGCCAGATGAGCGCT6490.15254902606026274No Hit
TCCCAGAGGATGTCCTGAAGAAACTTCCCCAGGGCCTGGCTGTGCTGCTG6370.14972839691893275No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT6030.14173661435183119No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5880.1382108279251687No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC5860.1377407230682804No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC5770.13562525121228292No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5390.12669325893140465No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT5270.12387262979007467No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA5160.12128705307718887No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4980.11705610936519392No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG4940.11611589965141725No Hit
GTCGTGGGAGTTGCTGCAGAAGCAAGCGAAAAGCCAGATGAGCGCTTCTA4850.11400042779541976No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG4840.11376537536697562No Hit
CTTCTAAACTTAGAGAGAGGTGAGTAAGGCTTGTGGGCGATCACTTGTCG4840.11376537536697562No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG4770.11212000836786647No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT4540.10671380251365066No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC4520.10624369765676234No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA4390.1031880160869882No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4340.10201275394476739No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)