FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002152311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210888
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG14160.6714464549903266Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10300.48841091005652293Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCACCCCAGCGCTGAAAGG7150.33904252494214937No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT6100.2892530632373582Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTATACCCCTCCGCTGGGGCAAAATTTACGCCCCAGCGAGTAGGCAGGCGTGGAGGTCCGTGACGAAGCCTTG5830.2764500587989833No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA4960.23519593338644207No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC4960.23519593338644207Clontech Universal Primer Mix Long (96% over 26bp)
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGA4950.23472174803687265No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTTCTTCAAGCGG4870.23092826524031712No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGTATTCAGTTTTATGAGGTAAAGCGAAT4850.2299798945411783No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACTTA4830.2290315238420394No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4800.22760896779333104Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG4730.22428967034634498No Hit
ATCCTAAGAGATAGGGTAATTCCGTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGAAAGGGAAGCCGGTTAAAAT4690.2223929289480672No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT4560.21622851940366453No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC4290.20342551496528966Clontech Universal Primer Mix Long (96% over 26bp)
GGTATAAGTAACACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACACTCCT4220.20010621751830357No Hit
ATCCTGATGTCTTCGGAAGGATTTGAGTAAGAGCATGGCTGTTGGGACCCGAAAGATGGTGAACTATGCTTGAAT4140.19631273472174804No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTCAAACAGCACGTGAAATTGTTGAAAGGGAAG4080.1934676226243314No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3980.188725769128637Clontech Universal Primer Mix Long (96% over 26bp)
GTCTAGATGAACCAACACCTTTTGTGGTGTCTGATGAGCGTATATTCCGGCACTTTAACCCCTCGTTCGGTTCAT3840.18208717423466486No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACCAACACCTTTTGTGGTGTCTGAT3830.18161298888509542No Hit
CTACTAGATAGTTCGATTAGTCTTTCGCCCCTATGCCCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCG3720.17639695003983158No Hit
GTTGGACACGGGTCAATCGATCCTAAGAGATAGGGTAATTCCGTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGA3660.17355183794241494No Hit
CATCAGGATCGGTCGATGGTGCACCCTTGCGGGTTCCCACCTCCGTTCACTTTCATTACGCGCTCGGGCTTGACA3620.17165509654413716No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG3620.17165509654413716Clontech Universal Primer Mix Long (96% over 26bp)
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCA3600.1707067258449983No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACTTATACCCCTCCG3560.16880998444672055No Hit
GTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTT3520.16691324304844277No Hit
CCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCTGGGGCGTAAATTTTGCCCCAGCGGAGGGGTAT3340.15837790675619287No Hit
ACCCGGGGTCACCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCTGGGGCGTAAATTTTGCCCCAG3340.15837790675619287No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC3300.1564811653579151No Hit
GTTCTGACGTGCAAATCGATCGTCAAATTTGGGCATAGGGGCGAAAGACTAATCGAACTATCTAGTAGCTGGTTC3250.15411023861006792No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGTCTGCACCCCAG3210.15221349721179014No Hit
GAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTTGGTGCGCTCCCGACGACCCTTGAAAATC3090.14652327301695686No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT3070.145574902317818No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC3070.145574902317818No Hit
GCCGAAGCCTGCCAAGGGTGTCTGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCA3060.14510071696824856No Hit
CTTCCAGCCATTGAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACTGTTAAGAAGAAAAGAGAACTCT2990.14178141952126247No Hit
GTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACTTATACCCCTC2970.1408330488221236No Hit
GCCTTATAGGGGAGACGACATACTACCAGCCTGGACTGAGGTCCGCGCATCTGCTAGGATGCTGGCGTAATGGCT2940.13941049277341527No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC2930.13893630742384583Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGATAGCAGTAACGTATTCAGTTTTATGAGGTAAAGCGAATGATTAGAGGCCTGGGGGTTGAAACAACCTTCACC2920.13846212207427638No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT2920.13846212207427638Clontech Universal Primer Mix Long (96% over 26bp)
GGCTGGAAGAGCGCTGCACTTTTGCGGCGTTTGGTGCGCTCCCGACGACCCTTGAAAATCCGCTTGAAGAAATAG2890.13703956602556808No Hit
GCACTAGAGGCCGTTCGACCCGGGGTCACCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCTGGGG2880.13656538067599863No Hit
CCACAAAAGGTGTTGGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAAC2870.1360911953264292No Hit
GGGTAATTCCGTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGAAAGGGAAGCCGGTTAAAATTCCGGCATCTGGA2840.13466863927772088No Hit
TCTGTAGGCAGGCCAGCATCAGTTTGGGCGGTAGGATAAAGGTCTCTGTCACGTACCTCCTTTCGGGGAGGCCTT2770.1313493418307348No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2760.13087515648116535Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGACAGACTGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAACGTCTCCGCTCTCAGTTGCGTTGCC2750.1304009711315959No Hit
GAGTACGACCTGGCGTGAAAACTATTTCTTCAAGCGGATTTTCAAGGGTCGTCGGGAGCGCACCAAACGCCGCAA2740.12992678578202646No Hit
GTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCATTTGAGTATTTGCTACTAC2730.12945260043245704No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC2680.12708167368460985Clontech Universal Primer Mix Long (96% over 26bp)
CATCAGACACCACAAAAGGTGTTGGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA2680.12708167368460985No Hit
TGCCAAGGGTGTCTGCACCCCAGCGCTGAAAGGCGCCCACCTTGCGGTTAGTGCCCTTCTGCGCCAGAGGCTTCT2680.12708167368460985No Hit
CCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACT2670.1266074883350404No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTTC2660.12613330298547096No Hit
GGGTTAAAGTGCCGGAATATACGCTCATCAGACACCACAAAAGGTGTTGGTTCATCTAGACAGCAGGACGGTGGC2640.1251849322863321No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2630.12471074693676265Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTT2600.12328819088805432No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA2600.12328819088805432No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG2600.12328819088805432Clontech Universal Primer Mix Long (96% over 25bp)
GTCCGGAGCTAGGGTTCAATGGCTGGAAGAGCGCTGCACTTTTGCGGCGTTTGGTGCGCTCCCGACGACCCTTGA2570.121865634839346No Hit
CGTCAGAACCGCTGCGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAAGCATAGTTCACCATCTTTCG2520.1194947080914988No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTTCTTCAAGCGGATTTTCAAGGGTCGT2500.11854633739235992No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT2490.11807215204279048No Hit
CTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACTAGATAGTTCGATTAGTCTTTCGCCCCTATGCCCAAA2490.11807215204279048No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCG2470.11712378134365162No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATGTACCTCCCATTTAGAGCTGCATT2400.11380448389666552No Hit
ATTCAAGCATAGTTCACCATCTTTCGGGTCCCAACAGCCATGCTCTTACTCAAATCCTTCCGAAGACATCAGGAT2400.11380448389666552No Hit
CGTTATTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGAACGAGGGGTTAAAGTGCCGGA2380.11285611319752666No Hit
GTTGAATGAAACGTTATTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGAACGAGGGGTT2330.11048518644967945No Hit
CTAGAGGCCGTTCGACCCGGGGTCACCCCCAAGGCTTCGTCACGGACCTCCACGCCTGCCTACTCGCTGGGGCGT2330.11048518644967945No Hit
CTAATAACGTTTCATTCAACTGTCCACGTTCAATTAAGTAACAAGGACTTCTTACATATTTAAAGTTTGAGAATA2330.11048518644967945No Hit
GTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACCAACACCTTTTGTGG2260.10716588900269337No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG2260.10716588900269337No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTAC2240.10621751830355448No Hit
CTCTTAGGATCGATTGACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTC2240.10621751830355448No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG2140.10147566480786009No Hit
CAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCC2110.10005310875915178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)