FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002152329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218463
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCGACTTTCGTCTTTGCAGAGTGCTGTGTTTTTAATAAACAG4930.22566750433711888No Hit
CTACTAAGGGTGTACGGTGAATGCCTTGGCACCAGGAGTCGATGAAGGACGCGATAAGCTGCGATAAGCCCCGGT4790.2192590965060445No Hit
GTACTGGTTCACTATCGGTCACCAGGAAGTATTTAGCCTTACGAGATGGTCCTCGTAGCTTCACACGGGATTTCT4580.20964648475943296No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4260.19499869543126297Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG3770.17256926802250266Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTCTAAGGCGCTTGAGAGAACTCTGGAGAAGGAACTCGGCAAATTGACACCGTAACTTCGGAAGAAGGTGTGCC3700.16936506410696547No Hit
ACACAGCACTCTGCAAAGACGAAAGTCGACGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGG3650.16707634702443894No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT3560.16295665627589112No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.1620411694428805No Hit
ACTCAGGAACCTGTCCAGCAAGCCTCTCCCTTGTCATTTACCGGGCTGTCACCGTCTGTGGCCGATCGTTCCAGA3260.14922435378073173No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA3180.14556240644868926No Hit
ATCCTACACAGATCTGTTCAAAGTCCAATGTAAAGCTACAGTAAAGGTTCATGGGGTCTTTCCGTCTTTCCGCGG2970.1359497947020777No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGACTGAAGTCCCAGTAAACGGCGGCCGTAACAATAACGGT2910.13320333420304584No Hit
GTTCCAGATCGTTCAATTAAGAGAGAGGTTGATCACTTGCCGGCCTTGCGGCCTAACAGGCCCTACAACCCCAGC2900.13274559078654052No Hit
GTGCTGTGTTTTTAATAAACAGTCGCAGCCACCGATTCTCTGCGGCCTCATTGGGCTCCCCAAGTAAATGGTTCA2870.13137236053702459No Hit
GTGCATATACGGCGCCTTGTGCGGCTTGGCATGCACCTGTGTTTTTGCTAAACAGTCGCTTGAGCCGATTCACTG2770.12679492637197146No Hit
GTTATGGCTAGGGGGTCATGGCGACTTACCAAACCATTGCAAACTCCGAATACCGATGAGTACAGCTTGGGAGAC2760.12633718295546614No Hit
CCCTATACGTCGACTTTCGTCTTTGCAGAGTGCTGTGTTTTTAATAAACAGTCGCAGCCACCGATTCTCTGCGGC2590.11855554487487585No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGTTACGGC2590.11855554487487585No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAGTCAAGAGCTTGCACCCCATCATTTAATCTT2580.11809780145837052No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC2560.11718231462535991No Hit
ACTAAGGGTGTACGGTGAATGCCTTGGCACCAGGAGTCGATGAAGGACGCGATAAGCTGCGATAAGCCCCGGTGA2490.11397811070982271No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2190.10024580821466336Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)