FastQCFastQC Report
Wed 8 May 2024
EGAF00002152347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361610
Total Bases27.1 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27230.7530212106966069Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22000.6083902546942839Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTT17220.47620364481070765No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCT16380.4529741987223805No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGA16160.44689029617543763No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14610.4040264373219767No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG14410.398495616824756Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA13630.376925416885595No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGG10770.29783468377533806No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAAC10430.2884322889300628No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAA10330.2856668786814524No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTT10000.2765410248610381No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG9920.2743286966621498Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC9890.27349907358756675Clontech SMART CDS Primer II A (96% over 26bp)
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATC9690.267968253090346No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGC9680.2676917120654849No Hit
CCCCAAGTCCGTGCCAGTTCTGAATTGATTGTTAATTGATAGTCGTTATA9680.2676917120654849No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT9510.2629905146428473Clontech Universal Primer Mix Long (96% over 26bp)
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAA9500.26271397361798626No Hit
TTCCAGCACCTGGTGGAGGACGCTCTTGGCAGGGTTCGCCCGTCCGGCGC9470.2618843505434031No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9220.2549708249218771No Hit
GTGTCAACACCCGTTAGGGCCATCACAATGCTTTGTTTTAATTAGACAGT8850.24473880700201875No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC8650.239207986504798Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG8570.23699565830590968Clontech Universal Primer Mix Long (96% over 26bp)
AAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT8570.23699565830590968No Hit
GGTTGGCACTAGCCTCACGGCCGGCGCCTTCCAGCACCTGGTGGAGGACG8480.23450678908216033No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCA8360.23118829678382785No Hit
GTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCC8230.22759326346063435No Hit
TCTTACGACAATCCCTAAAAAATTTTAGCAAGAAAGTTCCACAATTGGCT8220.22731672243577333No Hit
GTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCAC8180.2262105583363292No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGG8120.22455131218716298No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGA7910.21874395066508118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7870.217637786565637No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA7790.2154254583667487No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCG7690.21266004811813832No Hit
CTATTTACCCAGAACGAGCACATAAACCATATTATTGCTTCCCAATCAAG7340.202981112248002No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGTT7050.19496142252703189No Hit
CTCTAAGGGTTGGGTGCGTTGGGCCTTGAGCAGAAGCCCTGGGAGCAGGT6980.19302563535300463No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC6650.18389978153259035Clontech Universal Primer Mix Long (96% over 26bp)
GCTCTAAGGGTTGGGTGCGTTGGGCCTTGAGCAGAAGCCCTGGGAGCAGG6570.18168745333370206No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA6570.18168745333370206No Hit
GACTAGAAGAGCATGACATTTACTGTTGTGTCGATATTTTCTCCTCGGAC6270.17339122258787093No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTC6150.17007273028953845No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAA6110.16896656619009431No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT6080.1681369431155112Clontech Universal Primer Mix Long (100% over 23bp)
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCAGGT6060.1675838610657891No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC6050.16730732004092808Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTC6010.16620115594148394No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTT6010.16620115594148394No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATT5980.16537153286690082No Hit
GTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTT5970.16509499184203977No Hit
TGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTA5950.1645419097923177No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTG5910.16343574569287353No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5890.16288266364315146No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5730.15845800724537484Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAAATAGAGATCCTTATGGTTCTTGTTCCCCGGATAGTAGTTATGTA5430.1501617764995437No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGG5420.14988523547468266No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT5420.14988523547468266Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.1457371201017671No Hit
ATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAA5260.14546057907690604No Hit
CATAACTACTATCCGGGGAACAAGAACCATAAGGATCTCTATTTACCCAG5150.14241862780343464No Hit
TCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCAAGTCCGTGCCA4960.1371643483310749No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGT4850.1341223970576035No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAG4800.1327396919332983No Hit
GTCTCTAGTCGATAGAATAATGTAGGTAAGGGAAGTCGGCAAATTAGATC4740.13108044578413205No Hit
GCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAA4730.13080390475927103No Hit
CGTTAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCA4670.1291446586101048No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAA4610.12748541246093856No Hit
TTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTT4590.12693233041121652No Hit
ATCACATTGTGTCAACACCCGTTAGGGCCATCACAATGCTTTGTTTTAAT4570.12637924836149442No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC4500.12444346118746716Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4500.12444346118746716No Hit
CTCTTGGCAGGGTTCGCCCGTCCGGCGCACGCTTAACAACCAACTTAGAA4300.1189126406902464No Hit
GAATAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGAT4280.11835955864052432No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGC4180.11559414839191393No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA4150.11476452531733083No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4140.11448798429246979No Hit
AGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTT4120.11393490224274772No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG4070.11255219711844251No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG4030.11144603301899837Clontech Universal Primer Mix Long (96% over 26bp)
GTAGTTATGTAGCCAATTGTGGAACTTTCTTGCTAAAATTTTTTAGGGAT3970.10978678686983213No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3950.10923370482011005No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTA3950.10923370482011005No Hit
GGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCC3780.10453250739747241No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGTTAGGGCC3770.10425596637261138No Hit
AAATCACATTGTGTCAACACCCGTTAGGGCCATCACAATGCTTTGTTTTA3750.1037028843228893No Hit
GGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCAAGTCCG3690.10204363817372308No Hit
GCCAAGAGCGTCCTCCACCAGGTGCTGGAAGGCGCCGGCCGTGAGGCTAG3680.10176709714886202No Hit
GGATTGGCTCTAAGGGTTGGGTGCGTTGGGCCTTGAGCAGAAGCCCTGGG3670.101490556124001No Hit
CATTTACTGTTGTGTCGATATTTTCTCCTCGGACCTTGAAAATTTATGGT3670.101490556124001No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.101490556124001No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCT3660.10121401509913996No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)