FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002152351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521830
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG125032.395991031562003Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG102861.9711400264453942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT52391.0039668091140792Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC41220.7899124235862254Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG38750.7425790008240232Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA30200.5787325374164Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG27010.517601517735661Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC25820.49479715616196845Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT25680.49211429009447516Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG25410.4869401912500239Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC24410.4677768621965008Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG24070.46126133031830285Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20460.3920817124350842No Hit
CCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCAC18130.34743115574037525No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC17860.34225705689592395Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG17600.33727459134200793Clontech Universal Primer Mix Long (96% over 25bp)
ATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACTGTGGCAGCTC17440.33420845869344423No Hit
TCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCA15450.29607343387693313No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC14890.2853419696069601Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT14620.2801678707625088Clontech SMART CDS Primer II A (96% over 26bp)
GTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTT12800.24529061188509668No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC12450.23858344671636358Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12230.23436751432458847No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC11530.22095318398712224Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10590.20293965467681044Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10310.197573922541824Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9900.18971695762987947Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9600.1839679589138225No Hit
AGCCTGTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGT9230.17687752716401892No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG9140.17515282754920186Clontech Universal Primer Mix Long (96% over 26bp)
ATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCC9060.17361976122492No Hit
GTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAA8500.162888296954947No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC8460.1621217637928061Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT8170.15656439836728436Clontech Universal Primer Mix Long (96% over 25bp)
CTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTGTTCTATCGACT8080.15483969875246728No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA7760.14870743345533985Clontech Universal Primer Mix Long (96% over 26bp)
CGTATGCACCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTG7650.1465994672594523No Hit
TACCTACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAA7600.14564130080677615No Hit
GTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAAAGGGACACGCAAATTCTCAATAGCCTGTACCGAATCCG7530.14429986777302953No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTT7440.14257516815821245No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT7270.1393174022191135Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG7250.13893413563804305Clontech Universal Primer Mix Long (96% over 26bp)
CACCATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAG7210.1381676024759021No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCT7020.1345265699557327Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA7010.13433493666519747Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT6800.1303106375639576Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA6670.1278194047869996Clontech Universal Primer Mix Long (96% over 26bp)
GCTCTGAAGACTGTGGCAGCTCGGGCTTTACAATATGCATTCGCTTCACGGTGAATGTATATTAAGTCTGTAATG6590.12628633846271775No Hit
CTCATAGAGAAGCCAATGGGAGGTATCGGAAGAGTTTTCTTTTCTGTTTTACAATCGTATGCACCATGGAAGTCT6550.1255198053005768No Hit
GATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAG6520.12494490542897113No Hit
ATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCG6370.12207040607094263No Hit
TGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATG6360.12187877278040742No Hit
CCATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAG6340.12149550619933694No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA6320.12111223961826648Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6100.1168963072264914Clontech Universal Primer Mix Long (96% over 26bp)
TAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCAC6050.11593814077381522No Hit
CTCCTTGGACCTTGAAAATCTATGAAAGGGACACGCAAATTCTCAATAGCCTGTACCGAATCCGCAGCAGGTCTC5900.11306364141578674No Hit
GTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCT5890.11287200812525153No Hit
GTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCACCATTACAGACTTAAT5680.10884770902401167No Hit
AAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAAC5650.10827280915240596No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC5620.10769790928080027Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGCAG5580.10693137611865933Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTG5500.10539830979437748No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT5500.10539830979437748Clontech Universal Primer Mix Long (96% over 26bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC5460.10463177663223655Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA5420.10386524347009561Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACTGTGGC5390.10329034359848994No Hit
TCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAA5360.10271544372688424No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAG5310.10175727727420808No Hit
GTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCG5300.10156564398367285No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT5260.10079911082153192Clontech Universal Primer Mix Long (96% over 26bp)
CTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCCAATCCTTA5230.10022421094992623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)