FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002152358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170719
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACTCGGCCATGGGCGCAAGTGCGAAGCTCCCATGAGTAGGAGGGCGTGGGTGTCGTGGCGCAGCCTGCG10060.5892724301337285No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9140.5353827049127514Clontech Universal Primer Mix Long (96% over 26bp)
AGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT8550.5008229898253856No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG8420.4932081373485084Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCAGATAGGATCCGTCCGGTTGCCCAAACAGTTCCCGTCCAGTCCGATGGCTTCTGACTAAGGCCCGACGTAC5480.3209953197945161No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT4860.28467833105864027Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCCTGTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAGGGTTAAGATTCCCTTACCTGGACAAGGC4440.2600764999794985No Hit
CCCCTAGGCCCAACCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCATTTTGCCGACTTCCCTTATCTACA4290.25129013173694786No Hit
ATACAGGATTGCCCCATCTCTTAGGATCGACTAACCCATGTCCAATTGCTGTTCACATGGAACCTTTCTCCACTT4170.24426103714290737No Hit
GATATAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCA3660.2143873851182352No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.21380162723539853No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACT3510.20560101687568463No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCTGGCAAGATCGGAAATGCTCCCCCGGA3380.1979861643988074No Hit
GTCTATATCAACCAACACCCTTTGTGGGATCTAGGTTAGCGCGCAGTTTGGCACCTTAACTCGGCTTTCGGATCA3120.18275645944505298No Hit
GCGCTGAAGCGCGCGACCTATACTCGGCCATGGGCGCAAGTGCGAAGCTCCCATGAGTAGGAGGGCGTGGGTGTC3080.18041342791370615No Hit
TATCTACATTGTTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCTGGCAAGA3040.17807039638235933No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2850.16694099660846184Clontech Universal Primer Mix Long (96% over 26bp)
GTCCAGGTAAGGGAATCTTAACCCTTTTCCCTTTCGATGGGCGGCGCGAATCGCGCCCTTGATACAGGATTGCCC2820.16518372295995173No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2760.16166917566293149Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2750.16108341778009477Clontech Universal Primer Mix Long (96% over 26bp)
GAGTAGGAGGGCGTGGGTGTCGTGGCGCAGCCTGCGGCGTGAGCCGGGGTGAAACGGCACCTAGTGCAGATCTTG2700.15815462836591124No Hit
GTTCTGAGTTGGTTGTTCGTCGCCTAGGGAGCGGCGTGAGCCTTTCTCCGCCCGTCCGCCGGCGGCACGCGGCCG2670.15639735471740113No Hit
CTCCTACTCATGGGAGCTTCGCACTTGCGCCCATGGCCGAGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCG2650.1552258389517277No Hit
ATCCTAAGAGATGGGGCAATCCTGTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAGGGTTAAGATT2610.15288280742038085No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC2580.15112553377187074Clontech Universal Primer Mix Long (96% over 26bp)
CTCAATGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGATCCGAAAGCCGAGTTAA2550.14936826012336063No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG2550.14936826012336063Clontech Universal Primer Mix Long (96% over 26bp)
AACCTAGATCCCACAAAGGGTGTTGGTTGATATAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGG2450.14351068129499353No Hit
GTGCCAAACTGCGCGCTAACCTAGATCCCACAAAGGGTGTTGGTTGATATAGACAGCAGGACGGTGGTCATGGAA2440.14292492341215682No Hit
GCTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGGTATGCTCGTACTCAAACCTTTCACAGAAGATCATGGT2370.13882461823229986No Hit
GTGCTTTACCAGCTGCTGAGCCCTATCTCCGGATGATCCGATTCCAGGGCCTTCGAGCTGTTAAAAAGAAAAGAG2320.13589582881811632No Hit
GTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT2280.13355279728676947No Hit
ATCTCCGGATGATCCGATTCCAGGGCCTTCGAGCTGTTAAAAAGAAAAGAGAACTCTTCCCAGGGCCACCGCCGG2250.13179552363825936No Hit
TACCTGGACAAGGCGGCTGACGGCAACGTAAGCGAGCCCGGAGACGCCGGCGGTGGCCCTGGGAAGAGTTCTCTT2170.12710946057556569No Hit
CCCCTTGTCCGTACCAGTTCTGAGTTGGTTGTTCGTCGCCTAGGGAGCGGCGTGAGCCTTTCTCCGCCCGTCCGC2140.12535218692705558No Hit
CTATAGAAGCGACCTCTGTGTCGGACCGGGCCCAAGTTCCCTGGAAAGGGACGTCGCAGAGGGTGAGAACCCCGT2130.12476642904421886No Hit
TCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGGTTGTTCGTCGCCT2120.12418067116138215No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCGACCTATACTCGGCCA2020.11832309233301508No Hit
GTCCTCGACAGTCCTTGAAAATCCAAGGGAACGAATAATCTTTGCGCCTGGTCGTACTCATAACCGCAGCAGGTC1980.11598006080166824No Hit
ATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGTTGGGCCTAGGG1970.11539430291883153No Hit
GAGTACGACCTGGCAAGATCGGAAATGCTCCCCCGGATTTTCAAGGACCGTCGGTGGCGCGCCGGACACCGCGAG1920.112465513504648No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCCCAGGATAGCTGGAGCTCGGTCAGTTTTATCGGGTAAAGCGAAT1910.11187975562181127No Hit
GTACTTGTTCGCTATCGGTCTCCCGCCAGTATTTAGCCTTAGATGGGATTTACCACCTGCTTAGGGCTGCATTCC1890.11070823985613787No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1880.11012248197330116Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT1860.10895096620762774Clontech Universal Primer Mix Long (96% over 26bp)
GTTTGGCACCTTAACTCGGCTTTCGGATCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGC1860.10895096620762774No Hit
GCCTCTAGTCGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCT1860.10895096620762774No Hit
CTCTTAGGATCGACTAACCCATGTCCAATTGCTGTTCACATGGAACCTTTCTCCACTTCAGTCTTCAAAGTTCTC1850.10836520832479103No Hit
TCCCAGGGCCACCGCCGGCGTCTCCGGGCTCGCTTACGTTGCCGTCAGCCGCCTTGTCCAGGTAAGGGAATCTTA1850.10836520832479103No Hit
TCCGGAGATAGGGCTCAGCAGCTGGTAAAGCACCGCACGTCTCGCGGTGTCCGGCGCGCCACCGACGGTCCTTGA1810.10602217679344421No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1780.10426490314493408Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.10426490314493408No Hit
TCTTAGGATCGACTAACCCATGTCCAATTGCTGTTCACATGGAACCTTTCTCCACTTCAGTCTTCAAAGTTCTCA1770.10367914526209737No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1760.10309338737926066Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACTCGGCCATGGGCGCAAGTGCGAAGCTCCCATGAGTAGGAGGGCGTGGGTGTCGTGGCGCAGCCTGCGGCGT1740.10192187161358723No Hit
GTGCCCGCACCATCGACCGACCATGATCTTCTGTGAAAGGTTTGAGTACGAGCATACCTGTTGGGACCCGAAAGA1730.10133611373075053No Hit
GTACATGGGTTCGACCTGAGATCAGACAAGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAA1710.10016459796507711No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)