FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002152359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170719
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACTCGGCCATGGGCGCAAGTGCGAAGCTCCCATGAGTAGGAGGGCGTGGGTGTCGTGGCGCAGCCTGCG8760.5131239053649564No Hit
AGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCT6920.40534445492300214No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG6340.3713704977184731Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG6260.3666844346557794Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCAGATAGGATCCGTCCGGTTGCCCAAACAGTTCCCGTCCAGTCCGATGGCTTCTGACTAAGGCCCGACGTAC4840.2835068152929668No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT3590.21028707993837828Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCCTGTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAGGGTTAAGATTCCCTTACCTGGACAAGGC3360.19681464863313397No Hit
GATATAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCA3110.18217070156221626No Hit
GCGCTGAAGCGCGCGACCTATACTCGGCCATGGGCGCAAGTGCGAAGCTCCCATGAGTAGGAGGGCGTGGGTGTC3020.1768988806166859No Hit
CCCCTAGGCCCAACCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCATTTTGCCGACTTCCCTTATCTACA2960.17338433331966566No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACT2650.1552258389517277No Hit
ATCCTAAGAGATGGGGCAATCCTGTATCAAGGGCGCGATTCGCGCCGCCCATCGAAAGGGAAAAGGGTTAAGATT2590.15171129165470745No Hit
ATACAGGATTGCCCCATCTCTTAGGATCGACTAACCCATGTCCAATTGCTGTTCACATGGAACCTTTCTCCACTT2590.15171129165470745No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2570.15053977588903403Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCAAACTGCGCGCTAACCTAGATCCCACAAAGGGTGTTGGTTGATATAGACAGCAGGACGGTGGTCATGGAA2540.14878250224052392No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2280.13355279728676947Clontech Universal Primer Mix Long (96% over 26bp)
AACCTAGATCCCACAAAGGGTGTTGGTTGATATAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGG2270.13296703940393279No Hit
GAGTAGGAGGGCGTGGGTGTCGTGGCGCAGCCTGCGGCGTGAGCCGGGGTGAAACGGCACCTAGTGCAGATCTTG2240.13120976575542265No Hit
GTCTATATCAACCAACACCCTTTGTGGGATCTAGGTTAGCGCGCAGTTTGGCACCTTAACTCGGCTTTCGGATCA2240.13120976575542265No Hit
GCTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGGTATGCTCGTACTCAAACCTTTCACAGAAGATCATGGT2210.12945249210691254No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCTGGCAAGATCGGAAATGCTCCCCCGGA2150.12593794480989226No Hit
TATCTACATTGTTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCTGGCAAGA2140.12535218692705558No Hit
GTTCTGAGTTGGTTGTTCGTCGCCTAGGGAGCGGCGTGAGCCTTTCTCCGCCCGTCCGCCGGCGGCACGCGGCCG2120.12418067116138215No Hit
CTCCTACTCATGGGAGCTTCGCACTTGCGCCCATGGCCGAGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCG1960.11480854503599482No Hit
TACCTGGACAAGGCGGCTGACGGCAACGTAAGCGAGCCCGGAGACGCCGGCGGTGGCCCTGGGAAGAGTTCTCTT1950.1142227871531581No Hit
CTATAGAAGCGACCTCTGTGTCGGACCGGGCCCAAGTTCCCTGGAAAGGGACGTCGCAGAGGGTGAGAACCCCGT1920.112465513504648No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1890.11070823985613787Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGTTCGCTATCGGTCTCCCGCCAGTATTTAGCCTTAGATGGGATTTACCACCTGCTTAGGGCTGCATTCC1870.10953672409046444No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCGACCTATACTCGGCCA1840.10777945044195432No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCCCAGGATAGCTGGAGCTCGGTCAGTTTTATCGGGTAAAGCGAAT1810.10602217679344421No Hit
CCCCTTGTCCGTACCAGTTCTGAGTTGGTTGTTCGTCGCCTAGGGAGCGGCGTGAGCCTTTCTCCGCCCGTCCGC1800.10543641891060748No Hit
ATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGTTGGGCCTAGGG1780.10426490314493408No Hit
TCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGGTTGTTCGTCGCCT1770.10367914526209737No Hit
GTGTCAACATCCTTTGGGACCATCACAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTT1750.10250762949642395No Hit
GTGCTTTACCAGCTGCTGAGCCCTATCTCCGGATGATCCGATTCCAGGGCCTTCGAGCTGTTAAAAAGAAAAGAG1730.10133611373075053No Hit
CTCAATGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGATCCGAAAGCCGAGTTAA1730.10133611373075053No Hit
GTTTGGCACCTTAACTCGGCTTTCGGATCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGC1710.10016459796507711No Hit
GTCCTCGACAGTCCTTGAAAATCCAAGGGAACGAATAATCTTTGCGCCTGGTCGTACTCATAACCGCAGCAGGTC1710.10016459796507711No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)