FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002152380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288867
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG22150.7667888682334777Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG20070.6947834124354807Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15300.5296555162064203No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT13070.4524573592691446Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT10860.3759515624837728No Hit
GTTCTAAGTTGGTTGTTAATTGTACGCCGGACGCCCGAAAGCTGCCAAGGCCGTCAGAACCCCGATGCTGGAAGC10570.3659123402811674No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT9890.3423720951164377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9690.3354484935973995No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG9320.3226398307871789No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG9050.3132929687364774Clontech Universal Primer Mix Long (96% over 26bp)
CTTGTGCGCCGTCCCTCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATGTGGATTCTCCACGGCAACGTAA8990.3112158882807659No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC8560.2963301450148338Clontech Universal Primer Mix Long (96% over 26bp)
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC8320.288021823191988No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA8030.27798260098938266Clontech Universal Primer Mix Long (96% over 26bp)
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGA8000.27694406076152694No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7760.26863573893868115Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC7700.26655865848296967Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC7640.26448157802725825No Hit
GCTCCAAGGACGTCGCAACCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCT7070.24474931369799943No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTCCTTCCAGCGG7060.24440313362204752No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACGCCGGACGCCCGAAAGCTGCCAAGGCCGTCAGAA6630.22951739035611546No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACGCCGGACGCCCGAAAGCTGCCAAGGCCGTCAGAACCCCGA6490.22467086929278873No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT6490.22467086929278873No Hit
GCCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAACGGTTGGTAGAGCCTGACACCTTTGTCAGGTCC5990.20736186549519328No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC5750.1990535436723475Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5710.19766882336853986No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5410.18728342108998258Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACGCCGGACGCCCG5330.1845139804823673No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACGCCGGACGCCCGAAAGCTGCCAAGGC5300.1834754402545116No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA5280.1827830801026078No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTCC5120.17724419888737725No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5030.17412857820381006Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG5030.17412857820381006No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG5020.17378239812785815No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT4850.1678973368366757No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA4830.16720497668477188No Hit
CTCCACGGCAACGTAATTGAAAGCGGAGACGGCGGCGGGGGCCCTGGGAAGAGTTCTCTTTTCTTCTTAACAGCC4790.16582025638096426No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4770.16512789622906043Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTAC4560.15785811463407035No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT4550.15751193455811843Clontech Universal Primer Mix Long (96% over 26bp)
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA4500.1557810341783589No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCTCCACCAGGTGCTGGAAGG4480.1550886740264551No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA4380.151626873266936No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG4320.14954979281122455No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT4220.14608799205170545No Hit
GCCTTGGGAGTGATCCCGGGTCGAACAGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAAC4110.14228001121623446No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAA4050.140202930760523No Hit
GAGTACGACCTGGCGTGAAAACTATTCCTTCCAGCGGATTTTCAAGGGACGTCGAGAGCGCACCGGACCTGACAA4010.13881821045671539No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.13708731007695585No Hit
AGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG3920.1357025897731482No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG3890.13466404954529246Clontech Universal Primer Mix Long (96% over 25bp)
CTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAA3790.13120224878577338No Hit
ATCCTAAGAGATAGGGAAACTCCGTTTTAAAGTGCGCACTTGTGCGCCGTCCCTCGAAAGGGAAGCCGGTTAATA3770.13050988863386956No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3740.12947134840601385Clontech Universal Primer Mix Long (96% over 26bp)
TTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTG3730.12912516833006193No Hit
GAGCTAGGGTTTAACGGTTGGTAGAGCCTGACACCTTTGTCAGGTCCGGTGCGCTCTCGACGTCCCTTGAAAATC3630.12566336757054286No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCC3580.1239324671907833No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTT3510.12150920665911995No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTCCTTCCAGCGGATTTTCAAGGGACGT3410.11804740589960086No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGC3380.11700886567174514No Hit
GTGATAGGCTGTTAAGAAGAAAAGAGAACTCTTCCCAGGGCCCCCGCCGCCGTCTCCGCTTTCAATTACGTTGCC3340.1156241453679375No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC3320.11493178521603369Clontech Universal Primer Mix Long (96% over 26bp)
GCGCACAAGTGCGCACTTTAAAACGGAGTTTCCCTATCTCTTAGGATCGACTAACCCATGTCCAACTGCTGTTCA3310.11458560514008176No Hit
GTTTTAAAGTGCGCACTTGTGCGCCGTCCCTCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATGTGGATTC3280.11354706491222605No Hit
CCTTTGTCAGGTCCGGTGCGCTCTCGACGTCCCTTGAAAATCCGCTGGAAGGAATAGTTTTCACGCCAGGTCGTA3260.11285470476032222No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACT3190.11043144422865886No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTA3140.10870054384889932No Hit
CCTTGGAGCAGGTCGCCACTAGCCTTCTGGCCGGCGGCTTCCAGCATCGGGGTTCTGACGGCCTTGGCAGCTTTC3120.1080081836969955No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT3110.10766200362104361Clontech SMART CDS Primer II A (96% over 26bp)
GGCTAGTGGCGACCTGCTCCAAGGACGTCGCAACCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCC3100.10731582354509168No Hit
CTCTAAGGGTTGGGTACGTTGGGCCTTGGTTGCGACGTCCTTGGAGCAGGTCGCCACTAGCCTTCTGGCCGGCGG3090.10696964346913979No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT3070.10627728331723597No Hit
ATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCC3020.10454638293747641No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCCATATTTAGCTTTAGATGAAATTTACCACCCATTTTGAGCTGCATT3020.10454638293747641No Hit
CTACCAACCGTTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGGCTGTTAAGAAGAAAAGAGAACTCT2990.1035078427096207No Hit
CCGTTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGGCTGTTAAGAAGAAAAGAGAACTCTTCCCAGG2990.1035078427096207No Hit
GGTTGGTAGAGCCTGACACCTTTGTCAGGTCCGGTGCGCTCTCGACGTCCCTTGAAAATCCGCTGGAAGGAATAG2900.10039222202605351No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)