FastQCFastQC Report
Wed 8 May 2024
EGAF00002152381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002152381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288867
Total Bases21.6 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27130.9391865460575282Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20260.701360833878567Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA14440.49988402967455614No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG13660.47288198375030727Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12100.41887789190180946No Hit
GTTCTAAGTTGGTTGTTAATTGTACGCCGGACGCCCGAAAGCTGCCAAGG11700.4050306888637331No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10940.37872100309138806Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10270.3555269380026102Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9990.34583389587595675No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATC9900.3427182751923896No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT9820.3399488345847743No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG9580.3316405127619285Clontech Universal Primer Mix Long (96% over 26bp)
CTTGTGCGCCGTCCCTCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGG9340.32333219093908266No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC8820.3053308269895834Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT8150.28213676190080555Clontech Universal Primer Mix Long (96% over 26bp)
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGA8110.28075204159699796No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTG7940.27486698030581547No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACGCCGGACGC7870.2724437197741521No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGG7830.2710589994703445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7820.27071281939439257No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7640.26448157802725825No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA7560.26171213741964294No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTG7220.24994201483727807No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTT7220.24994201483727807No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTC7170.2482111144575185No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA6690.23159447081182688No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACGCCGGACGCCCGAAA6160.21324692678637575No Hit
GCCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAACGGTTGGT6130.21220838655852No Hit
GCTCCAAGGACGTCGCAACCAAGGCCCAACGTACCCAACCCTTAGAGCCA6020.20840040572304902No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGC5980.2070156854192414No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC5930.20528478503948183Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA5860.2028615245078185No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC5860.2028615245078185Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT5790.20043826397615513Clontech Universal Primer Mix Long (100% over 23bp)
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAG5620.19455320268497267No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATTGTACG5580.19316848238116502No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT5570.19282230230521313Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5310.1838216203304635No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGG5190.17966745941904058No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTT5160.17862891919118487No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG5130.17759037896332913No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5130.17759037896332913Clontech Universal Primer Mix Long (96% over 26bp)
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT5100.17655183873547342No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAA4950.1713591375961948No Hit
CTCCACGGCAACGTAATTGAAAGCGGAGACGGCGGCGGGGGCCCTGGGAA4900.16962823721643525No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGA4890.16928205714048333No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA4500.1557810341783589No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.15301159357074362No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4370.15128069319098408No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4360.15093451311503217No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTA4300.14885743265932072No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTT4140.14331855144409017No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAA4100.14193383114028255No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAA4090.14158765106433063No Hit
CCTTGGAGCAGGTCGCCACTAGCCTTCTGGCCGGCGGCTTCCAGCATCGG4080.14124147098837872No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCT4000.13847203038076347No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC3970.13743349015290773Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG3840.13293314916553292Clontech Universal Primer Mix Long (96% over 26bp)
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGT3800.1315484288617253No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3710.12843280817815814No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCT3610.12497100741863904No Hit
GAGTACGACCTGGCGTGAAAACTATTCCTTCCAGCGGATTTTCAAGGGAC3560.12324010703887947No Hit
TTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTT3540.12254774688697566No Hit
AGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTT3510.12150920665911995No Hit
GAGCTAGGGTTTAACGGTTGGTAGAGCCTGACACCTTTGTCAGGTCCGGT3400.11770122582364896No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA3310.11458560514008176No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCT3300.11423942506412986No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3280.11354706491222605No Hit
CCTTTGTCAGGTCCGGTGCGCTCTCGACGTCCCTTGAAAATCCGCTGGAA3280.11354706491222605No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG3260.11285470476032222Clontech Universal Primer Mix Long (96% over 26bp)
GTCCGGAGCTAGGGTTTAACGGTTGGTAGAGCCTGACACCTTTGTCAGGT3260.11285470476032222No Hit
CTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAAC3210.11112380438056267No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAA3200.11077762430461077No Hit
ATCCTAAGAGATAGGGAAACTCCGTTTTAAAGTGCGCACTTGTGCGCCGT3170.10973908407675503No Hit
GGTTGGTAGAGCCTGACACCTTTGTCAGGTCCGGTGCGCTCTCGACGTCC3100.10731582354509168No Hit
GTTTTAAAGTGCGCACTTGTGCGCCGTCCCTCGAAAGGGAAGCCGGTTAA3100.10731582354509168No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTC3100.10731582354509168No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACT3100.10731582354509168No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCCATATTTAGCTTTAGATGAAA3040.10523874308938024No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT3020.10454638293747641Clontech Universal Primer Mix Long (96% over 26bp)
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTT2990.1035078427096207No Hit
GCGCACAAGTGCGCACTTTAAAACGGAGTTTCCCTATCTCTTAGGATCGA2950.10212312240581306No Hit
ATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGA2950.10212312240581306No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA2950.10212312240581306Clontech SMART CDS Primer II A (96% over 26bp)
CTCTAAGGGTTGGGTACGTTGGGCCTTGGTTGCGACGTCCTTGGAGCAGG2940.10177694232986115No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA2940.10177694232986115Clontech Universal Primer Mix Long (96% over 26bp)
GTGATAGGCTGTTAAGAAGAAAAGAGAACTCTTCCCAGGGCCCCCGCCGC2940.10177694232986115No Hit
GCCTTGGGAGTGATCCCGGGTCGAACAGCCTCTAGTGCAGATCTTGGTGG2940.10177694232986115No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGG2930.10143076225390925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)